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Uas rhebrnai

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The UAS-RhebRNAi is a laboratory product designed for experimental use. It is a tool for the targeted knockdown of the Rheb gene expression in various model systems. The core function of this product is to facilitate the study of Rheb-related biological processes through RNA interference technology. No further details or interpretations are provided.

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Lab products found in correlation

Uas gfp, by BD (3 mentions) Uas rheb, by BD (3 mentions) Uas flp, by BD (2 mentions) Rn gal4, by BD (2 mentions) Pdm2 gal4, by BD (2 mentions) Frt42d, by BD (2 mentions) Frt42d ubi gfp, by BD (2 mentions) Tre gfp, by BD (2 mentions) Uas htt25q cerulean, by BD (2 mentions) Uas htt96q cerulean, by BD (2 mentions) Uas atg1rnai, by BD (2 mentions) Uas xrp1rnai, by BD (2 mentions) Rpl52d2, by BD (2 mentions) Rpl141, by BD (2 mentions) Rps131, by BD (2 mentions) Dp 2 3 cam14t, by BD (2 mentions) Tub gal4, by BD (2 mentions) Uas gfp mcherry atg8a, by BD (2 mentions) Uas gadd34, by BD (2 mentions) Uas luciferase rnai, by BD (1 mentions)

3 protocols using uas rhebrnai

1

Drosophila Genetic Stock Database

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All the genotypes analysed in this study are listed in Supplementary Table 1, in supplementary information. The following stocks were obtained from the Bloomington Drosophila Stock Center (BDSC), the Vienna Drosophila Resource Center (VDRC) or are described in Flybase: ci-GAL4 (Flybase #FBti0076751), hh-GAL4 (Flybase #FBti0017278), rn GAL4 (BDSC #7405), tub-GAL4 (BDSC #5138), pdm2-GAL4 (BDSC #49828), nub-GAL4 (Flybase #FBti0150342), FRT42D (BDSC #1802), FRT42D ubi-GFP (BDSC #5626), TRE-GFP (BDSC #59010), UAS-GFP (BDSC #35786), UAS-FLP (BDSC #4540), UAS-P35 (Flybase #FBtp0001646), UAS-HTT25Q Cerulean (BDSC #58360), UAS-HTT96Q-Cerulean (BDSC #56772) UAS-GFP-mCherry-Atg8a (BDSC #37749), UAS-Rheb (BDSC #9688), UAS-GADD34 (BDSC #76250), UAS-Atg1RNAi (BDSC #44034), UAS-RhebRNAi (BDSC #33966), UAS-Xrp1RNAi (BDSC #34521), UAS Rpn2RNAi (VDRC #106457), UAS-Rpt6RNA (VDRC #49244), RPL52d2 (BDSC #25907), RPL141 (BDSC #2247), RPS131 (BDSC #2246), Dp(2:3)Cam14T (BDSC #4519).
Recasens-Alvarez C., Alexandre C., Kirkpatrick J., Nojima H., Huels D.J., Snijders A.P, & Vincent J.P. (2021). Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition. Nature cell biology, 23(2), 127-135.
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2

Drosophila Genetic Stock Database

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the genotypes analysed in this study are listed in Supplementary Table 1, in supplementary information. The following stocks were obtained from the Bloomington Drosophila Stock Center (BDSC), the Vienna Drosophila Resource Center (VDRC) or are described in Flybase: ci-GAL4 (Flybase #FBti0076751), hh-GAL4 (Flybase #FBti0017278), rn GAL4 (BDSC #7405), tub-GAL4 (BDSC #5138), pdm2-GAL4 (BDSC #49828), nub-GAL4 (Flybase #FBti0150342), FRT42D (BDSC #1802), FRT42D ubi-GFP (BDSC #5626), TRE-GFP (BDSC #59010), UAS-GFP (BDSC #35786), UAS-FLP (BDSC #4540), UAS-P35 (Flybase #FBtp0001646), UAS-HTT25Q Cerulean (BDSC #58360), UAS-HTT96Q-Cerulean (BDSC #56772) UAS-GFP-mCherry-Atg8a (BDSC #37749), UAS-Rheb (BDSC #9688), UAS-GADD34 (BDSC #76250), UAS-Atg1RNAi (BDSC #44034), UAS-RhebRNAi (BDSC #33966), UAS-Xrp1RNAi (BDSC #34521), UAS Rpn2RNAi (VDRC #106457), UAS-Rpt6RNA (VDRC #49244), RPL52d2 (BDSC #25907), RPL141 (BDSC #2247), RPS131 (BDSC #2246), Dp(2:3)Cam14T (BDSC #4519).
Recasens-Alvarez C., Alexandre C., Kirkpatrick J., Nojima H., Huels D.J., Snijders A.P, & Vincent J.P. (2021). Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition. Nature cell biology, 23(2), 127-135.
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3

Genetic Manipulation of Drosophila Metabolism

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Flies were maintained at 25 ℃ and 50% humidity on a standard cornmeal food (the recipe for 1 L food is: 56 g cornmeal, 40 g yeast, 88 g glucose, 10 g agar, and 1.25 g nipagin) with a 12-h light/dark cycle. The Gal4/UAS (Upstream Activating Sequence) binary system [16 (link)] was applied to manipulate transgene expression in various tissues, with experimental crosses conducted at 25 ℃. FLP-out strains were crossed with indicated UAS-transgene strains at 25 ℃ to generate clones labeled with fluorescence. 5–7-day-old mated flies or 3rd instar larvae were used in all experiments, with detailed descriptions provided in the corresponding figure legends.
Fly strains utilized in this study: w1118 (lab stock), lpp-Gal4 (lab stock), hsFLP; AyGal4, UAS-GFP (lab stock), hsFLP; AyGal4, UAS-RFP (lab stock), UAS-CG33474-3×Flag (this study), CG33474-Gal4 (this study), Ubi-GFP-PTS1 (this study), UAS-GFP (BDSC, #52262), UAS-RFP (BDSC, #8545), UAS-Luciferase-RNAi (BDSC, #31603), UAS-lacZ (lab stock), UAS-CG33474-RNAi (BDSC, #64672), UAS-Rheb-RNAi (BDSC, #33966), UAS-Pex19-RNAi (VDRC, #22064), UAS-bmm-RNAi (VDRC, #37877), UAS-Sod1-RNAi (BDSC, #34616), UAS-Sod2-RNAi (BDSC, #25969), UAS-Rheb (BDSC, #9688), and UAS-GFP-PTS1 (BDSC, #64248).
Liu M, & He L. (2024). Dietary cysteine and methionine promote peroxisome elevation and fat loss by induction of CG33474 expression in Drosophila adipose tissue. Cellular and Molecular Life Sciences: CMLS, 81(1), 190.
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