QNM peak force AFM experiments were carried out on a multimode 8-nanoscope V instrument (Bruker, CA) under pH 6.0 buffer using an MSCT cantilever with its calibrated spring constant designed between 0.01 to 0.03 N/m and peak force setpoints of 100–200 pN; 5 μl drops of fresh cages +/− auxilin, ATP and Hsc70 or Hsc70ΔC were deposited on freshly peeled mica and imaged under the same buffer following routine optimization for biological AFM. Data were analyzed with instrument software (Nanoscope ver8.15, Bruker, CA) and exported as ascii files for further analysis with Excel (Microsoft, Richmond, WA), and displayed with ImageJ (ver 1.4x, NIH, Bethesda, MD). Samples as described for AFM were absorbed for 20 seconds to a Formvar-carbon grid, followed by a rinse and 20 second incubation with 1% uranyl acetate. Grids were wicked, air dried, and examined at 25,000× magnification in a JEOL JEM 1200EX-II.
Multimode 8 nanoscope 5 instrument
Manufactured by Bruker
The Multimode 8-nanoscope V instrument is a scanning probe microscope designed for high-resolution imaging and analysis of nanoscale materials and surfaces. It is capable of various scanning probe microscopy techniques, including atomic force microscopy (AFM), scanning tunneling microscopy (STM), and related modes. The instrument provides the ability to characterize the topography, mechanical, electrical, and other properties of samples at the nanometer scale.
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Lab products found in correlation
2 protocols using multimode 8 nanoscope 5 instrument
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Cages Dynamics via AFM and Cryo-EM
2
Cages Dynamics via AFM and Cryo-EM
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QNM peak force AFM experiments were carried out on a multimode 8-nanoscope V instrument (Bruker, CA) under pH 6.0 buffer using an MSCT cantilever with its calibrated spring constant designed between 0.01 to 0.03 N/m and peak force setpoints of 100–200 pN; 5 μl drops of fresh cages +/− auxilin, ATP and Hsc70 or Hsc70ΔC were deposited on freshly peeled mica and imaged under the same buffer following routine optimization for biological AFM. Data were analyzed with instrument software (Nanoscope ver8.15, Bruker, CA) and exported as ascii files for further analysis with Excel (Microsoft, Richmond, WA), and displayed with ImageJ (ver 1.4x, NIH, Bethesda, MD). Samples as described for AFM were absorbed for 20 seconds to a Formvar-carbon grid, followed by a rinse and 20 second incubation with 1% uranyl acetate. Grids were wicked, air dried, and examined at 25,000× magnification in a JEOL JEM 1200EX-II.
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