Nitrilotriacetic acid (Ni2+-NTA)-chelating Sepharose and Superdex 200 were obtained from GE Healthcare. N-dodecylphosphocholine (DPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (PC), 1,2-dioleoyl-sn-glycero- 3-phospho-l-serine (PS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′-bisphosphate) (PI(4,5)P2), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(biotinyl) (biotin-PE), 1,2-dipalmitoyl-sn-glycero-3-phospho-ethanolamine-N-(7-nitro- 2-1,3-benzoxadiazol-4-yl) (NBD-PE) and N-(lissamine rhodamine B sulfonyl)-1,2- dipalmitoyl-sn-glycero-3-phosphoethanolamine (rhodamine-PE) were obtained from Avanti Polar Lipids. 2-Sulfonatoethyl methanethiosulfonate sodium salt (MTSES) was purchased from Biotium. All other chemicals were from Sigma.
Nitrilotriacetic acid ni2 nta chelating sepharose
Manufactured by GE Healthcare
Nitrilotriacetic acid (Ni2+-NTA)-chelating Sepharose is a solid-phase chromatographic medium. It is used for the purification of recombinant proteins containing a histidine-tag.
Automatically generated - may contain errors
Lab products found in correlation
1 palmitoyl 2 oleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (3 mentions)
1 2 dioleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (3 mentions)
N lissamine rhodamine b sulfonyl 1 2 dipalmitoyl sn glycero 3 phosphoethanolamine rhodamine pe, by Avanti Polar Lipids (2 mentions)
1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions)
Superdex 200, by GE Healthcare (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
N dodecylphosphocholine, by Avanti Polar Lipids (1 mentions)
Brain l α phosphatidylinositol 4 5 bisphosphate, by Avanti Polar Lipids (1 mentions)
E coli cardiolipin, by Avanti Polar Lipids (1 mentions)
Superdex 200 increase 10 300 gl, by GE Healthcare (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dil, by Thermo Fisher Scientific (1 mentions)
Superose 6 increase 10 300 gl, by GE Healthcare (1 mentions)
1 2 dioleoyl sn glycero 3 phospho 1 rac glycerol, by Avanti Polar Lipids (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphoethanolamine n 7 nitro 2 1 3 benzoxadiazol 4 yl, by Avanti Polar Lipids (1 mentions)
3 protocols using nitrilotriacetic acid ni2 nta chelating sepharose
1
Lipid-Detergent Protein Purification Protocol
2
Reconstitution of Membrane Proteins
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1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (DGS-NTA), 1,2-dioleoyl-sn-glycero-3-phosphocholine (PC), 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (PS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE), brain L-α-phosphatidylinositol-4,5-bisphosphate (PI), and E.coli cardiolipin (CL) were obtained from Avanti Polar Lipids. Nitrilotriacetic acid (Ni2+-NTA)-chelating Sepharose and Superdex 200 increase 10/300 GL were purchased from GE Healthcare. All other chemicals were acquired from Sigma.
3
Lipid Membrane Reconstitution Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1,2-dioleoyl-sn-glycero-3-phosphocholine (PC), 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (PS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (PG), 1,2-dipalmitoyl-sn-glycero-3-phospho-ethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) and N-(lissamine rhodamine B sulfonyl)-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (rhodamine-PE) were obtained from Avanti Polar Lipids. Nitrilotriacetic acid (Ni2+-NTA)-chelating Sepharose and Superose 6 increase 10/300 GL were purchased from GE Healthcare. 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate) (Dil), N,N′-Dimethyl-N-(Iodoacetyl)-N′-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Ethylenediamine (IANBD amide) and Oregon GreenTM 488 (OG) maleimide were obtained from ThermoFisher. All other chemicals were acquired from Sigma.
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