Jurkat lucia nfat cd32

The CHO-S cell line was obtained from Thermo Fisher Scientific (Waltham, MA, USA) cat. no. R80007. Caco-2, and A549 cell lines were purchased from the Russian collection of vertebrate cell lines (Saint Petersburg, Russia). Jurkat-Lucia™ NFAT-CD16 cells and Jurkat-Lucia™ NFAT-CD32 were obtained from InvivoGen (San Diego, CA, USA) cat. code jktl-nfat-cd16 and jktl-nfat-cd32, respectively.

HEK293T, Raji and RAW 264.7 cells were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA). HEK293T/hACE2 cell was obtained from Creative Diagnostics (Shirley, NY, USA). Cells were cultured in DMEM or RPMI1640 supplemented with 10% heat-inactivated fetal bovine serum (FBS) and antibiotics-antimycotics at 37°C in a 5% CO₂ incubator. HEK-Blue™ hACE2-TMPRSS2, 293-hMyD88, Jurkat-Lucia™ NFAT-CD16 and Jurkat-Lucia™ NFAT-CD32 cells were purchased from InvivoGen (San Diego, CA, USA) and were cultured according to the suppliers’ protocol. HEK293T cells were transfected with SARS-CoV-2 spike (Wuhan)-His/pCMV3 vector (Sino Biological, Beijing, China) by using polyethylenimine (PEI; Polysciences, Warrington, PA, USA).