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Cytosponge ffpe clot

Manufactured by Qiagen
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The Cytosponge™ FFPE clot is a laboratory equipment product designed to assist in the processing of formalin-fixed, paraffin-embedded (FFPE) tissue samples. It provides a standardized method for the collection and recovery of tissue from FFPE clots. The core function of this product is to facilitate the extraction and preparation of FFPE tissue samples for downstream analysis, such as molecular testing or histological examination.

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Lab products found in correlation

Deparaffinization buffer, by Qiagen (2 mentions) Qubit dsdna hs assay kit, by Thermo Fisher Scientific (2 mentions) Qiaamp dna ffpe tissue kit, by Qiagen (2 mentions)

2 protocols using cytosponge ffpe clot

1

Extracting DNA from FFPE Cytosponge Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was extracted from 8 × 10μm sections of the processed Cytosponge™ FFPE clot using Deparaffinization Buffer (Qiagen) and the QIAamp FFPE DNA Tissue Kit (Qiagen). The protocol was followed as described by the manufacturer with the exception that samples were incubated at 56°C for 24 hours instead of the described 1 hour, and 10 μl of extra Proteinase K was added to the samples roughly half way through the 24 hour incubation. After extraction, DNA was quantified using the Qubit™ dsDNA HS Assay Kits (Invitrogen)
Weaver J.M., Ross-Innes C.S., Shannon N., Lynch A.G., Forshew T., Barbera M., Murtaza M., Ong C.A., Lao-Sirieix P., Dunning M.J., Smith L., Smith M.L., Anderson C.L., Carvalho B., O’Donovan M., Underwood T.J., May A.P., Grehan N., Hardwick R., Davies J., Oloumi A., Aparicio S., Caldas C., Eldridge M.D., Edwards P.A., Rosenfeld N., Tavaré S, & Fitzgerald R.C. (2014). Ordering of mutations in preinvasive disease stages of esophageal carcinogenesis. Nature genetics, 46(8), 837-843.
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2

Extracting DNA from FFPE Cytosponge Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was extracted from 8 × 10μm sections of the processed Cytosponge™ FFPE clot using Deparaffinization Buffer (Qiagen) and the QIAamp FFPE DNA Tissue Kit (Qiagen). The protocol was followed as described by the manufacturer with the exception that samples were incubated at 56°C for 24 hours instead of the described 1 hour, and 10 μl of extra Proteinase K was added to the samples roughly half way through the 24 hour incubation. After extraction, DNA was quantified using the Qubit™ dsDNA HS Assay Kits (Invitrogen)
Weaver J.M., Ross-Innes C.S., Shannon N., Lynch A.G., Forshew T., Barbera M., Murtaza M., Ong C.A., Lao-Sirieix P., Dunning M.J., Smith L., Smith M.L., Anderson C.L., Carvalho B., O’Donovan M., Underwood T.J., May A.P., Grehan N., Hardwick R., Davies J., Oloumi A., Aparicio S., Caldas C., Eldridge M.D., Edwards P.A., Rosenfeld N., Tavaré S, & Fitzgerald R.C. (2014). Ordering of mutations in preinvasive disease stages of esophageal carcinogenesis. Nature genetics, 46(8), 837-843.
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