Daudi cells were adsorbed on polyLysine-treated slides and fixed with Formalin (Sigma) containing 1% Triton-X-100. Cells were labeled with anti-Vpr antibody followed by labeling with a mixture of Alexa488-conjugated donkey anti-goat and anti-rabbit IgG (H + L) antibodies (Life Technologies). Nuclei were stained with DAPI staining solution from Sigma Aldrich. Cells were mounted on glass slides covered with anti-fade medium (Hardset Vectashield). Two-color images were obtained with a light microscope, Leica DC250 (Leica) with a Plan Apo 63 ×/1.32–0.6 oil-immersion objective lens. Digital images were processed with the ImageJ software (NIH Image). Percentages of Vpr+ Daudi B-cells were determined by using the Cell Counting plugin of ImageJ. A minimum of 750 cells were counted per 6-well plate.
Anti rabbit igg h l antibodies
Manufactured by Thermo Fisher Scientific
Anti-rabbit IgG (H + L) antibodies are secondary antibodies used to detect and bind to rabbit primary antibodies. They recognize the heavy and light chains of rabbit immunoglobulin G (IgG) molecules. These antibodies are commonly used in various immunoassays and immunochemical techniques for the identification and quantification of rabbit antigens.
Automatically generated - may contain errors
2 protocols using anti rabbit igg h l antibodies
1
Immunofluorescent Detection of Vpr in Daudi Cells
2
Daudi B-Cell Vpr Localization Analysis
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Daudi cells were adsorbed on polyLysine-treated slides and xed with Formalin (Sigma) containing 1% Triton-X-100. Cells were labeled with anti-Vpr antibody followed by labeling with a mixture of Alexa488conjugated donkey anti-goat and anti-rabbit IgG (H+L) antibodies (Life Technologies). Nuclei were stained with DAPI staining solution from Sigma Aldrich. Cells were mounted on glass slides covered with anti-fade medium (Hardset Vectashield). Two-color images were obtained with a light microscope, Leica DC250 (Leica) with a Plan Apo 63 × /1.32-0.6 oil-immersion objective lens. Digital images were processed with the ImageJ software (NIH Image). Percentages of Vpr + Daudi B-cells were determined by using the Cell Counting plugin of ImageJ. A minimum of 750 cells were counted per 6-well plate.
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