Oxaliplatin

Human CRC cell line SW480 were purchased from the Chinese Academy of Science, Shanghai Institute of Biochemistry and Cell Biology. SW480 cells were maintained in Dulbecco’s modified eagle medium, high glucose (Thermo Fisher Scientific, USA) supplemented with 1% penicillin and streptomycin (Thermo Fisher Scientific, USA) and 10% fetal bovine serum (FBS; Gibco, NY) in a humidified atmosphere of 5% CO₂ at 37°C. Oxaliplatin-resistant CRC cell lines (SW480/R) were induced by Oxaliplatin (Meilun, China). Generally, the Oxaliplatin-resistant cell lines were developed from SW480 cells by stepwise exposure to increasing concentrations of Oxaliplatin from 0.2 to 2 µM, and the drug-resistant cell lines SW480/R was established after 6 months.

Two CRC cell lines (HCT116 and HT29) were bought from the Shanghai Cell Bank of the Chinese Academy of Sciences and cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 100 µg/mL of penicillin- streptomycin. Oxaliplatin (Qilu Pharmaceutical Co., Ltd in Jinan, China) was dissolved in DMSO at a concentration of 30 mol/L and the cells were treated by Oxaliplatin for 24 h. CCK8 assay kit (Meilun Co.,Ltd, Nanjing, China’s) was used to detect the vitality of the cells according to the manufacturer’s instructions. Briefly, 2 × 10³ of cells were plated in 96 well plates. After treatment with DMOS or Oxaliplatin, cells were incubated in 10% CCK8 reagent for 2 h. The OD value was measured at 450 nm with TECAN Infinite 200 PRO Plate reader. Total RNA was extracted using the TRIzol chemical. The primary strand DNA was created using the PrimeScript RT chemical agent Kit and gDNA tool (Invitrogen; Thermo Fisher Scientific, Inc.). Step-by-step RT-PCR procedures were carried out in accordance with the manufacturer’s instructions. The expression of lncRNAs was measured using the 2^−ΔΔCT method. In Supplemental Tables 1, the primers for the seven lncRNAs used in the RT-PCR test are listed.