N n dimethylformamide (dmf)

Manufactured by Scharlab

Sourced in Spain

N,N-dimethylformamide is a colorless, hygroscopic liquid. It is commonly used as a polar aprotic solvent in various chemical reactions and processes.

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Lab products found in correlation

14 protocols using n n dimethylformamide (dmf)

Hydrothermal Synthesis of MIL-101(Cr) Nanocrystals

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MIL-101(Cr) NPs were crystallized
following a hydrothermal synthesis procedure:^{24 (link)} 0.70 g of CrCl₃·6H₂O (≤ 98%, Sigma
Aldrich) and 0.45 g of terephthalic acid (98%, Sigma Aldrich) in 26
mL of deionized water. The obtained solution was heated at 180 °C
for 30 min in a microwave (Anton Paar, Multiwave 3000). The synthesized
nanocrystals were activated as follows: first, they were washed and
centrifuged at 10,000 rpm for 15 min with deionized water. Second,
the MOF NPs were treated with DMF (99.5%, Scharlau) at 200 °C
for 24 h. Finally, they were washed overnight with methanol (99.9%,
Scharlau) at 70 °C with two stages of washing and centrifugation
at 10,000 rpm for 15 min with methanol.

Echaide-Górriz C., Aysa-Martínez Y., Navarro M., Téllez C, & Coronas J. (2021). Polyamide-MIL-101(Cr) Thin Films Synthesized on Either the Outer or Inner Surfaces of a Polysulfone Hollow Fiber for Water Nanofiltration. ACS Applied Materials & Interfaces, 13(6), 7773-7783.

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SEC Analysis of Copolymer Molecular Weight

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HKT-based copolymer apparent average molecular weight (M_n and M_w) and polydispersity index (Ð) were determined by SEC, using a Perkin-Elmer Isocratic LC pump 250 coupled to a refraction index detector (Series 200). Two Resipore columns (250 mm × 4.6 mm, Varian, Palo Alto, CA, USA) were used as solid phase, degassed chromatographic-grade dimethylformamide (DMF, 0.7 mL/min, Scharlau, Barcelona, Spain) with LiBr (0.1% w/v) was used as eluent, and temperature was fixed at 70 °C. Monodisperse PMMA standards (Scharlau) with molecular weights between 10,300 and 1,400,000 Da were used to obtain the calibration curve. Data were analyzed using the Perkin-Elmer LC solution program.

Espinosa-Cano E., Aguilar M.R., Portilla Y., Barber D.F, & San Román J. (2020). Anti-Inflammatory Polymeric Nanoparticles Based on Ketoprofen and Dexamethasone. Pharmaceutics, 12(8), 723.

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Chlorophyll Extraction and Analysis Protocol

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Human α-amylase, porcine
pepsin, porcine bile, porcine pancreatin, salts, and the chemicals
needed for enzymatic determinations were supplied by Sigma-Aldrich
Chemical Co. (Madrid, Spain). Gastric lipase (RGE15) was provided
by Lipolytech (Marseille, France). high performance liquid chromatography
(HPLC)-grade solvents (acetone, methanol) were supplied by VWR BDH
Chemicals (Radnor), except for N,N-dimethylformamide (DMF), which was supplied by Scharlab (Barcelona,
Spain). The purified water was obtained from a Milli-Q water purification
system (Millipore, Milford, MA). Chlorophyll a and b and pheophytin a were purchased from
Sigma-Aldrich Chemical Co., and chlorin e₄ and rhodin g₇ were acquired from Frontier Sci (Utah). The rest of chlorophyll
standards were laboratory-produced following established protocols.^{31 (link),32 (link)}

Viera I., Herrera M, & Roca M. (2021). In Vitro Bioaccessibility Protocol for Chlorophylls. Journal of Agricultural and Food Chemistry, 69(31), 8777-8786.

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Photopolymer Resin Synthesis and Characterization

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Clear photopolymer standard resin (a mixture of proprietary acrylic monomers and oligomers and phenylbis (2,4,6-trimethyl benzoyl)-phosphine oxide as photoinitiator) was purchased from XYZprinting, Inc. (XYZprinting, New Taipei City, Taiwan). phenylbis (2,4,6-trimethyl benzoyl)-phosphine oxide and silver perchlorate (AgClO₄) were purchased from Alfa Aesar (Thermo Fisher, Kendal, Germany). Methanol, N,N-dimethylformamide (DMF), and isopropanol (IPA) were purchased from Scharlau (Scharlab, Sentmenat, Spain). All products were used as received.

Valencia L.M., Herrera M., de la Mata M., Hernández-Saz J., Romero-Ocaña I., Delgado F.J., Benito J, & Molina S.I. (2022). Stereolithography of Semiconductor Silver and Acrylic-Based Nanocomposites. Polymers, 14(23), 5238.

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Peptide Synthesis Using Fmoc-Protected Amino Acids

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All Fmoc-protected amino acids, Fmoc-Wang resins, DIC (N,N′-Diisopropylcarbodiimide), and Oxyma were purchased from Iris Biotech GmbH (Marktredwitz, Germany). The following amino acid side-chain-protecting groups were used: OtBu (Asp, Glu), tBu (Ser, Thr), Pbf (Arg), Trt (Gln, His), and Boc (Lys). Peptide-synthesis grade N,N-dimethylformamide (DMF) was purchased from Scharlau (Barcelona, Spain); acetonitrile (ACN) from Carlo Erba (Milan, Italy); dichloromethane (DCM), trifluoroacetic acid (TFA), piperidine were purchased from Sigma-Aldrich (Milan, Italy).

Nuti F., Gellini C., Larregola M., Squillantini L., Chelli R., Salvi P.R., Lequin O., Pietraperzia G, & Papini A.M. (2019). A Photochromic Azobenzene Peptidomimetic of a β-Turn Model Peptide Structure as a Conformational Switch. Frontiers in Chemistry, 7, 180.

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PNA Synthesis Using Rink Amide Resin

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All chemicals and reagents were of analytical grade. SFN (d,l-sulforaphane, 574215-25MG; Merck Millipore, Burlington, MA) was diluted in dimethyl sulfoxide (DMSO) (D8418; Sigma-Aldrich, St. Louis, MO) at final stock concentration of 150 mM. Stock aliquots of SFN were stored at -20°C, protected from the light, and diluted 1:10, at moment of use, in DMSO. All reactants and solvents for PNA synthesis were of analytical grade. Rink amide ChemMatrix Ò , Fmoc-glycine, acetic anhydride, and m-cresol were obtained from Sigma (St. Louis, MO). Piperidine, N,N-Diisopropylethylamine (DIPEA), and trifluoroacetic acid (TFA) were from Alfa Aesar (Haverhill, MA). N,N,N¢,N¢-Tetramethyl-O-(1H-benzotriazol-1-yl)uronium hexafluorophosphate (HBTU) was purchased from TCI Europe (Eschborn, Germany). N,N-Dimethylformamide (DMF) was from Scharlab (Barcelona, Spain). Fmoc-protected PNA monomers were purchased from LGC Link (Bellshill, Scotland).

Gasparello J., Gambari L., Papi C., Rozzi A., Manicardi A., Corradini R., Gambari R, & Finotti A. (2020). High Levels of Apoptosis Are Induced in the Human Colon Cancer HT-29 Cell Line by Co-Administration of Sulforaphane and a Peptide Nucleic Acid Targeting miR-15b-5p. Nucleic acid therapeutics, 30(3).

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Synthesis and Characterization of Hydrocaffeic Acid-grafted Polymers

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N-vinylcaprolactam (V) (Sigma-Aldrich, Saint Louis, MO, USA), 1,4-dioxane (Panreac, Barcelona, Spain), 3,4-dihydroxyhydrocinnamic acid or hydrocaffeic acid (HCA) (Sigma-Aldrich, Saint Louis, MO, USA), thionyl chloride (Scharlau, Barcelona, Spain), N,N-dimethylformamide (DMF) (Scharlau, Barcelona, Spain), toluene (Merck, Kenilworth, NJ, USA), dimethyl sulfoxide (DMSO), triethylamine (Scharlau, Barcelona, Spain), ethanol (VWR Chemicals, Radnor, PA, USA), phosphate buffered saline solution 10 mM (PBS, Arlington, TX, USA) (pH 7.4) (Sigma-Aldrich, Saint Louis, MO, USA) were used as received. 2-Hydroxyethyl methacrylate (H) (Fluka, now Sigma-Aldrich, Saint Louis, MO, USA) was previously purified according to the literature [64 (link)] and azobisisobutyronitrile (AIBN) (Fluka, now Sigma-Aldrich, Saint Louis, MO, USA) was previously crystallized in methanol (Sigma-Aldrich, Saint Louis, MO, USA).

Puertas-Bartolomé M., Vázquez-Lasa B, & San Román J. (2018). Bioactive and Bioadhesive Catechol Conjugated Polymers for Tissue Regeneration. Polymers, 10(7), 768.

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Solid-Phase Peptide Synthesis

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Fmoc-(D)-amino acids were purchased from Iris Biotech GmbH (Marktredwitz, Germany), while other reagents: 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethylaminium tetrafluoroborate (TBTU), N,N′-Diisopropylcarbodiimide (DIC), 4-(Dimethylamino)pyridine (DMAP), N,N-Diisopropylethylamine (DIPEA), triisopropylsilane (TIS) and Trifluoroacetic acid (TFA) were from Novabiochem (Merck, Darmstadt, Germany) and Sigma Aldrich (Merck, Darmstadt, Germany).
The resin (TentaGel^® M NH₂ Monosized Amino TentaGel Microspheres, M30352) was obtained from Rapp Polymere GmbH (Tübingen, Germany). Other peptide synthesis reagents (piperidine) and solvents were reagent grade from Scharlau (Barcelona, Spain): methanol, dichloromethane (DCM), and Carlo Erba (Milan, Italy) reagents: N,N-Dimethylformamide (DMF). Solvents for chromatography (acetonitrile) were analytical grade obtained from Scharlau (Barcelona, Spain).

Kalafatovic D., Mauša G., Rešetar Maslov D, & Giralt E. (2020). Bottom-Up Design Approach for OBOC Peptide Libraries. Molecules, 25(15), 3316.

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Synthesis and Characterization of Polymeric Nanoparticles

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k-CG (MW: 672 000 g mol⁻¹, CAS No. 11114-20-8, 15.3% sulphate content), APDMS (97%, MW: 191.34 g mol⁻¹, CAS No. 3179-76-8), PAA (MW: 516.68, generation 0.0, d = 0.854 g ml⁻¹l, CAS No. 155773-72-1), MTX (MW: 454.44 g mol⁻¹, CAS No. 59-05-2) and ribonuclease-A from bovine pancreas (MW: 13 700 g mol⁻¹, powder, CAS No. 9001-99-4) were procured from Sigma Aldrich (Darmstadt, Germany). SA (MW: 60 275 g mol⁻¹, food grade, purity 99.99%) was imported from Dabur, India Limited. PVA (MW: 65 000 g mol⁻¹, CAS No. 9002-89-5, polymerization degree 1500) was acquired from Duskan. In addition, m ethanol (MW: 32.04 g mol⁻¹, d = 0.79 g ml⁻¹), ethanol (MW: 46.07 g mol⁻¹, HPLC grade, d = 0.79 g ml⁻¹) and N,N-dimethylformamide (MW: 73.10 g mol⁻¹, 99.8%, d = 0.95 g ml⁻¹) were obtained from Scharlau, Spain. Sodium chloride (MW: 58.44 g mol⁻¹), sodium azide (MW: 65.01 g mol⁻¹), sodium hydroxide (MW: 40 g mol⁻¹), calcium chloride (anhydrous, MW: 110.98 g mol⁻¹) and potassium chloride (MW: 74.55 g mol⁻¹) were purchased from Daejung, Korea.

Anees Ur Rehman Qureshi M., Arshad N., Rasool A., Janjua N.K., Butt M.S., Naqeeb Ur Rehman Qureshi M, & Ismail H. (2024). Kappa-carrageenan and sodium alginate-based pH-responsive hydrogels for controlled release of methotrexate. Royal Society Open Science, 11(4), 231952.

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Preparation of Nc-Based Aqueous Solutions

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Photophysical properties of the NIR absorbing dyes were measured in either spectroscopic grade toluene [56] , [88] , [89] (link) (Scharlab, Spain) or in Milli-Q water (Merck Millipore, MA, USA). In order to solubilize the highly nonpolar Ncs in a water-based solution to mimic the in vivo environment, the Cremophor-EL (BASF, Germany) solution used by Bézière and Ntziachristos [71] (link) was employed. The final solution had a solvent composition of 2.5% toluene, 10% Cremophor, 1% 1,2-propanediol (Sigma–Aldrich), 1% N,N-dimethylformamide (Scharlab) and 85.5% PBS (Sigma–Aldrich, pH 7.4). The Nc was dissolved to 200 μM in toluene. The 2-propanediol and N,N-dimethylformamide were added and mixed by sonication for 5 min. Cremophor-EL was added and mixed by pipetting and 30 min of sonication. PBS was added and the mixture was sonicated for a further 30 min. All solutions were prepared at a final dye concentration of 5 μM. These solutions were used for the spectroscopy in this study.

Duffy M.J., Planas O., Faust A., Vogl T., Hermann S., Schäfers M., Nonell S, & Strassert C.A. (2018). Towards optimized naphthalocyanines as sonochromes for photoacoustic imaging in vivo. Photoacoustics, 9, 49-61.

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