Protein concentration was determined with a BCA assay kit (Thermo Scientific, Rockford, IL). Equal amounts of protein were boiled in Laemmli buffer (Bio-Rad, Bio-Rad, Richmond, CA, USA) and loaded onto 12% SDS-PAGE gel and transferred to a 0.2 µm polyvinylidene difluoride membrane (GE Healthcare, Boulder, CO). The membranes were incubated with appropriate primary antibodies for overnight at 4 °C and were then incubated with the appropriate secondary antibodies for 1 h. Detection was performed using the ECL Western blotting detection system (Thermo Scientific, Rockford, IL). The immunoblot was analyzed with a Bio-Rad imaging system (Versadoc 5000 MP Imaging System, Bio-Rad, Richmond, CA, USA).
Versadoc 5000 mp imaging system
Manufactured by Bio-Rad
Sourced in United States
The Versadoc 5000 MP Imaging System is a multipurpose imaging device designed for capturing high-quality images of various types of gels and blots. The system utilizes a CCD camera and a selection of optical filters to provide exceptional image quality and sensitivity. This product is suitable for a wide range of imaging applications, including DNA, RNA, and protein gel documentation, as well as chemiluminescent and fluorescent detection.
Automatically generated - may contain errors
Lab products found in correlation
Ecl western blotting detection system, by Thermo Fisher Scientific (6 mentions)
Laemmli buffer, by Bio-Rad (4 mentions)
Bca assay kit, by Thermo Fisher Scientific (4 mentions)
0.2 m polyvinylidene difluoride membrane, by GE Healthcare (3 mentions)
Pvdf membrane, by GE Healthcare (1 mentions)
Enhanced chemiluminescence, by Thermo Fisher Scientific (1 mentions)
Anti actin, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated donkey anti rabbit, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated donkey anti goat, by Santa Cruz Biotechnology (1 mentions)
Anti mcl 1, by Rockland Immunochemicals (1 mentions)
Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions)
7 protocols using versadoc 5000 mp imaging system
1
Quantifying Protein Expression via Western Blot
2
Western Blot Protein Quantification and Detection
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Protein concentration was determined with a BCA assay kit (23225; Thermo Scientific, Rockford, IL, USA). Equal amounts of protein were boiled in Laemmli buffer (Bio-Rad, Bio-Rad, Richmond, CA, USA) and loaded onto 12% SDS-PAGE gel and transferred to a 0.2 µm polyvinylidene difluoride membrane (GE Healthcare, Boulder, CO, USA). The membranes were incubated with appropriate primary antibodies overnight at 4 °C and were then incubated with the appropriate secondary antibodies for 1 h. Detection was performed using the ECL Western blotting detection system (Thermo Scientific, Rockford, IL, USA). The immunoblot was analyzed with a Bio-Rad imaging system (Versadoc 5000 MP Imaging System, Bio-Rad, Richmond, CA, USA).
3
Grx2 Protein Expression in Eye
4
Western Blot Analysis of Mcl-1 Protein
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Two hundred GV oocytes from approximately 8–10 Mcl-1cKO and Mcl-1+/+ females were collected and lysed in RIPA buffer. Lysates were separated by SDS-PAGE and then transferred on to a PVDF membrane. Blots were incubated with anti-Mcl-1 (Rockland Immunochemicals, 600-401-394S) or anti-actin (Santa Cruz Biotechnologies, sc-1616). Membranes were washed and then incubated with specific HRP-conjugated donkey anti-rabbit (Santa Cruz Biotechnologies) or HRP-conjugated donkey anti-goat (Santa Cruz Biotechnologies) and detected using enhanced chemiluminescence (Thermo Scientific, Burlington, ON, Canada) SuperSignal West Femto Chemiluminescent Substrate and then imaged on the VersaDoc 5000MP Imaging System (Bio-Rad, Mississauga, ON, Canada).
5
Quantitative Protein Analysis via Western Blot
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Protein concentration was determined with a BCA assay kit (Thermo Scientific, Rockford, IL). Equal amounts of protein were boiled in Laemmli buffer (Bio-Rad, Bio-Rad, Richmond, CA, United States) and loaded onto 12% SDS-PAGE gel and transferred to a 0.2 µm polyvinylidene difluoride membrane (GE Healthcare, Boulder, CO). The membranes were incubated with appropriate primary antibodies for overnight at 4°C and were then incubated with the appropriate secondary antibodies for 1 h. Detection was performed using the ECL Western blotting detection system (Thermo Scientific, Rockford, IL). The immunoblot was analyzed with a Bio-Rad imaging system (Versadoc 5000 MP Imaging System, Bio-Rad, Richmond, CA, United States).
6
Nrf2 Translocation Dynamics Analysis
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Cells were treated without and with RTA 408 for 0.5, 2, and 6 h. The cytoplasmic and nuclear fractions of each sample were obtained using the NE-PER Nuclear and Cytoplasmic Extraction Reagents from Thermo Scientific (Thermo Scientific, Rockford, IL). 60 μg of each sample was loaded onto a 12% SDS-PAGE gel and transferred onto a PVDF membrane. The membranes were incubated with Nrf2 antibody for overnight at 4 °C and were then incubated with the appropriate secondary antibodies for 1 h. β-actin and B23 were used as cytoplasmic and nuclear marker, respectively. Detection was performed using the ECL Western blotting detection system (Thermo Scientific, Rockford, IL). The immunoblot was analyzed with a Bio-Rad imaging system (Versadoc 5000 MP Imaging System, Bio-Rad, Richmond, CA, USA).
7
Protein Concentration Determination and Western Blot Analysis
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