ELISA kits were used to measure the concentrations of D-LA, ET (also known as lipopolysaccharide, LPS), L-FABP, i-FABP, DAO, TNF-α, IFN-γ, and IL-10 (CUSABIO, Wuhan, China) in intestinal lavage fluid, according to the manufacturer's instructions. The absorbance of each solution was determined at a wavelength of 450 nm. Assay sensitivities were determined as follows: 7.8 ng/ml for D-LA, 0.78 pg/ml for ET, 1.56 pg/ml for L-FABP, 0.312 ng/ml for i-FABP, 0.195 mIU/ml for DAO, 1.56 pg/ml for TNF-α, 0.156 pg/ml for IFN-γ, and 0.78 pg/ml for IL-10. Intra-assay and interassay coefficients were 8% and 10%, respectively, for D-LA, ET, L-FABP, i-FABP, DAO, TNF-α, IFN-γ, and IL-10.
I fabp
Manufactured by Cusabio
Sourced in China
I-FABP is a lab equipment product that functions as an intestinal fatty acid-binding protein. It is a small cytoplasmic protein involved in the transport and metabolism of fatty acids in the intestine.
Automatically generated - may contain errors
4 protocols using i fabp
1
Quantifying Intestinal Biomarkers via ELISA
2
Diagnostic Evaluation of Mycobacterial Infections
3
Mycobacterial Infection Diagnostics Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Specimens were examined using Ziehl–Neelsen staining, locally, within 24 hours and cultured for mycobacteria in liquid medium (MGIT; BD, Sparks, MD). Culture and drug susceptibility testing was performed at the National Health Laboratory Services laboratory in Umtata, South Africa. Venous lactate and glucose were measured using a validated point-of-care meter (Accutrend Plus; Roche Products, Roche Diagnostics Division, United Kingdom) according to manufacturers' instructions.20 (link),21 (link) The remainder of the venous blood was centrifuged; plasma was frozen and stored. Assays for HIV and CMV viral loads, LPS, I-FABP, and cryptococcal antigen (CrAg) were performed retrospectively on these plasma specimens at the University of Cape Town. HIV viral load was measured on the Abbott RealTime HIV-1 platform (Abbott Park, IL) and CMV viral load was measured on the Argene CMV R-gene platform (Verniolle, France). The lower limit of quantification for HIV was 40 copies per milliliter and for CMV was 150 copies per milliliter. CrAg was measured using a lateral flow assay (IMMY, Norman, OK). LPS was measured using the Limulus amebocyte lysate assay (using the QCL-1000 kit from Lonza, Walkersville, MD), with modifications as previously described.22 (link) Enzyme-linked immunosorbent assay was used to measure I-FABP (Cusabio, Wuhan, China).
4
Evaluating Intestinal Barrier Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of cytokines and intestinal barrier integrity biomarkers in blood plasma was tested using commercial ELISA kits for IL-1β, IL-6, trefoil factor 3 (TFF3), intestinal-fatty acid binding protein (I-FABP), and zonulin (CUSABIO, Wuhan, China) according to the manufacturer's protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!