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Ctad vacutainer tubes

Manufactured by BD
Sourced in United States

The CTAD Vacutainer tubes are blood collection tubes designed for the collection of blood samples. They contain a combination of citrate, theophylline, adenosine, and dipyridamole (CTAD) as anticoagulants. The core function of these tubes is to facilitate the collection and transportation of blood specimens for various laboratory analyses.

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2 protocols using ctad vacutainer tubes

1

Biomarker Quantification in Plasma and Serum

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ELISA was used to quantify soluble TNF receptor I (sTNFRI), sCD14 and sCD163 (Quantikine, R&D systems), as well as tissue Plasminogen Activator, soluble Thrombomodulin, and soluble Endothelial Protein C Receptor (Asserachrom, Stago) in plasma collected into EDTA Vacutainer tubes (Becton Dickinson) and frozen. CRP and immunoglobulins were measured by turbidimetry in serum collected into SST II Vacutainer tubes, and D-dimers in blood collected into CTAD Vacutainer tubes (Becton Dickinson). Blood triglyceride, high-density lipoprotein (HDL) cholesterol, and insulin levels were quantified in serum collected into SST II Vacutainer tubes (Becton Dickinson) after 8 hours of fasting.
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2

Plasma Biomarkers for Thrombosis

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For βTG, blood perfusate was collected in CTAD vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ, USA) after breaking the vacuum and left on ice for 20 min until centrifugation at 1430g for 20 minutes. For TXB2, blood perfusate was collected in EDTA vacutainer tubes (Becton Dickinson) supplemented with meclofenamate (10μg/mL) (Sigma-Aldrich) and immediately centrifuged at 514g for 15 minutes. Plasma was collected and stored at −80C until assayed. Assays for beta-thromboglobulin (βTG; Asserachrom β-TG, Diagnostica Stago, Asnieres, France) and thromboxane B2 (TXB2; TXB2 ELISA Kit, Cayman Chemicals, Ann Arbor, MI, USA) were performed according to manufacturer’s instructions. The normal reference ranges for human βTG and TXB2 are <50 IU/mL and 1–2 pg/mL respectively.
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