Mercury plus 300 mhz spectrometer

Optical rotation and circular dichroism was measured on a JASCO P-2000 polarimeter and JASC0 J-815 CD spectrometer in MeOH, respectively. IR spectra were recorded on a Bomem MB-Series spectrometer, in a 100 to 3500 cm⁻¹ spectral region, using KBr matrix HR-ESI-MS and spectrum was recorded in a ESI-MS Quattro LCZ (Micromass Manchester, UK). NMR spectra were recorded on a Varian Mercury Plus 300 MHz spectrometer (7.02 T), operated at 75 MHz for ¹³C and 300 MHz for ¹H and 2D NMR and a Bruker (Avance III) 400 MHz instruments. Column chromatography was carried out in Silica gel (70–230 mesh, Merck) for vacuum liquid chromatography (VLC) and Silica gel C-18 (HF Bondesil C-18, Agilent) for flash chromatography (FC). Thin-layer chromatography (TLC) was performed on precoated silica gel aluminum sheets (Kieselgel 60 F254, 0.20 mm, Merck, Darmstadt, Germany) and visualized in UV 254 or 365 nm.

Peptides were synthesized employing an Fmoc-based solid phase peptide synthesis strategy using 2-chlorotrityl chloride resin. Protected peptide (0.016 mmol) was lipidated by 1-hexadecanol (16C), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) or 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine (DHPE). All peptides were characterizated by high-resolution mass spectra (HR-MS) (Supplementary file 1). HR-MS were measured on a Thermo Orbitrap coupled to a Thermo Accela HPLC system using electrospray ionization (ESI).
The small nucleophiles, ethanolamine 1 and phosphoethanolamine 2 were purchased from Sigma. Other small nucleophiles, glycerophosphoethanolamine 8 and diacetyl glycerophosphoethanolamine 10 were synthesized as shown in Figure 1—figure supplement 1B. Subsequently, these compounds were characterizated by NMR and HR-MS (Supplementary file 2). ¹H NMR spectra were recorded on a Varian Mercury Plus 300 MHz spectrometer (300 MHz), a Bruker DRX 400 (400.13 MHz) spectrometer. NMR spectra were calibrated to the solvent signals of DMSO-d₆ (δ = 2.50 and 40.45 ppm). All reactions were carried out under an inert atmosphere in dry solvents unless otherwise noted.