Mouse anti β tubulin monoclonal antibody

Western blot analysis of NG2 was performed with 200 μg of protein extract using polyclonal antibodies to NG2 (1:500, Abcam, Cambridge, UK), αSMA (1:1000, Proteintech, Rosemount, IL, USA) and the appropriate IRDyeTM 800-conjugated secondary antibody (1:10,000). Signals were detected using the Odyssey Imaging System (LI-COR Biosciences, Lincoln, NE, USA) and analyzed with Odyssey software. Results were normalized relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (mouse anti-GAPDH monoclonal antibody, 1:1000; Sigma, St. Louis, MO, USA) or β-tubulin (mouse anti-β-tubulin monoclonal antibody, 1:1000; TransGen Biotech, Beijing, China) expression to correct for variations in protein loading and transfer.

Western blot for PDGFR-β was performed with 50 μg protein extract using monoclonal antibodies to PDGFR-β (1:1,000, Abcam, Cambridge, UK) and the appropriate IRDyeTM 800-conjugated secondary antibody (1:10,000). Signals were detected using the Odyssey Imaging System (LI-COR Biosciences, Lincoln, NE, USA) and analyzed with Odyssey software. Results were normalized relative to β-tubulin (mouse anti-β-tubulin monoclonal antibody, 1:2,000, Transgen Biotech, China) expression to correct for variations in protein loading and transfer.