7 nitroindazole 7 ni

The following drugs were used: Citicoline (Alborz Daru, Iran), PTZ (Sigma, UK), aminoguanidine (a selective iNOS inhibitor) (Sigma, USA), and 7-nitroindazole (7-NI) (a selective nNOS inhibitor) (Sigma, Canada). Citicoline, AG, and PTZ were dissolved in physiological saline. 7-NI was suspended in a 1% aqueous solution of DMSO. All injections were administered in a volume of 10 mL/kg. Appropriate vehicle controls were run for each experiment. In all experiments, citicoline, AG and 7-NI were administered intraperitoneally. To assess clonic seizure threshold and latency, PTZ was administered intravenous and intraperitoneal, respectively.

We used male young (age 4 weeks) and adult (age 10 weeks) Wistar rats. Young and adult rats were divided into three groups by the type of administered compounds. The first group of youngs was treated with 7-nitroindazole (7-NI, Sigma) diluted in drinking water in the dose of 10 mg/kg/day (n = 7). The second group of youngs was treated with N^G–nitro L–arginine methyl esther (L-NAME, Sigma) diluted in drinking water in the dose of 50 mg/kg/day (n = 7). The third group of young rats was the control group with pure drinking water (n = 7). The adult rats received the same treatment with 7-NI (n = 6), L-NAME (n = 5) and control groups (n = 6) as the young rats.
Both substances, 7-NI and L-NAME, were administered in young and adult rats continuously during 6 weeks. Body weight of rats, daily consumption of food and water were observed during the whole treatment period. Animals were placed in an air conditioned room at a constant temperature (24 °C) and humidity (45–60%) with a light regime of 12:12 h light / dark cycle (light phase from 6.00 to 18.00). They were fed standard pellet for rats and drinking water ad libitum. All animal experiments were performed in accordance the rules of the State Veterinary and Food Administration of the Slovak Republic and in accordance with the Institutional guidelines of the Slovak Academy of Sciences issued by its Animal Research and Care Committee.

The detailed procedures regarding stereotactic surgery and injection were previously described.^{17 (link)} Briefly, adult mice were anesthetized with a mixture of ketamine (100 mg kg⁻¹, ACE Surgical Supply, Brockton, MA, USA) and xylazine (10 mg kg⁻¹, Sigma-Aldrich, St Louis, MO, USA) and placed in a stereotactic apparatus (David Kopf Instruments, Tujunga, CA, USA). LVs or drugs such as 7-nitroindazole (7-NI, 10 μm, Sigma-Aldrich) or 8-hydroxy-2-dipropylaminotetralin hydrobromide (8-OH-DPAT, 5 μm, Sigma-Aldrich) were stereotactically delivered into both sides of the dentate gyrus (DG) of the hippocampus (2 μl; coordinates: 2.3 mm posterior to the bregma, 1.35 mm lateral to the midline and 2.3 mm below the dura)^{15 (link)} or the mPFC region (2 μl; coordinates: 1.8 mm anterior to the bregma, 0.8 mm lateral to the midline and 1 mm below the dura).^{18 (link)} The mice were recovered on a hot pad (37 °C) and returned back to their home cages.