Uas dpp

Fly lines w¹¹¹⁸, FRT82, Labial^RNAi (BL26753), UAS-mcherry (BL38245), UAS-Tkv^QD (BL36536), UAS-Dpp (BL1486), UAS-Ubx (BL911), Ubx¹ (BL529), Ubx^RNAi line 1 (BL31913), Ubx^RNAi line 2 (BL34993), Dad^RNAi (BL33759) were obtained from Bloomington Drosophila Stock Center. esg-Gal4, UAS-GFP was a gift from Shigeo Hayashi; FRT82B, Dad²¹² from Hannele Ruohola-Baker; Btl-Gal4^ts, UAS-GFP from Dirk Bohmann; Dad::nlsGFP from Georgios Pyrowolakis; Su(H)-GBE-lacZ from Sarah Bray; esg^tsF/O (esgGal4, tubG80ts, UAS-GFP; UAS-flp, act > STOP > Gal4) from Huaqi Jiang; UAS::Dad from Thomas Kornberg; NP1::Gal4 from Dominique Ferrandon; MARCM82 (hsFlp; tub-Gal4, UAS-GFP; FRT82, tubGal80) from Norbert Perrimon.
Flies were cultured on yeast/molasses-based standard fly food (Recipe: 10 L H2O, 138 g agar, 220 g molasses, 750 g malt extract, 180 dry yeast, 800 g corn flour, 100 g soy flour, 62.5 ml propionic acid, 20 g Methyl 4-Hydroxybenzoate, and 72 ml ethanol) at 25 °C with a 12 h light/dark cycle. For TARGET (tubGal80ts) experiments, flies were raised at 18 °C to allow Gal80 to inhibit Gal4, and 3–4 days after eclosion shifted to 29 °C to inhibit Gal80 and to allow Gal4 to drive UAS-linked transgene expression.

All fly stocks were raised in standard Bloomington medium at 25°C. The following fly stocks were used: nos-gal4 (Van Doren et al., 1998 (link)), cnn^HK21 (Megraw et al., 2001 (link)), and UAS-dpp (obtained from the Bloomington Drosophila Stock Center, Bloomington, IN); Ubi-Pavarotti-GFP and UAS-Pavarotti-GFP (Minestrini et al., 2002 (link); obtained from David Glover, University of Cambridge); cnn^mfs3 (Megraw et al., 1999 (link); obtained from Thom Kaufman, Indiana University); dsas-4^S2214 (Basto et al., 2006 (link); obtained from the Bloomington Stock Center); and UAS-Upd (Zeidler et al., 1999 (link)), Asl(asterless)-YFP (Varmark et al., 2007 (link)), and Ubi-Cnb-YFP (Januschke et al., 2011 (link); obtained from Cayetano Gonzalez, IRB Barcelona). To assess MR inheritance in cnn mutants, a cnn^HK21/CyO; Ubi-PavGFP/TM3 fly stock was generated, which was subsequently crossed with cnn^mfs3/CyO to obtain transheterozygous mutant flies (cnn^HK21/cnn^mfs3; Ubi-Pav-GFP/+), as well as control siblings (cnn/CyO; Ubi-Pav-GFP/+).