Xenolight d luciferin k salt bioluminscent substrate

Bioluminescent imaging via the IVIS (In vivo Imaging System) was utilized to track cell growth during the implantation period. Implanted 4T1 cells were labeled with firefly luciferin. Briefly, on the day of implantation and every 7 days during the time course of the study, 150 μL of 63 mM substrate (Xenolight D-Luciferin-K+ Salt Bioluminscent substrate, PerkinElmer, Waltham, USA) was injected intraperitoneally. After 10 min of incubation, mice were imaged. Similarly, to assess metastasis, collected organs and tumors on day of sacrifice were imaged by adding 28 μL of 63 mM substrate per mL of culture media. Images were processed and analyzed using LivingImage software (version 4.7.2).