Bacterial DNA was extracted using BioRobot EZ1 following the EZ1 DNA Tissue Kit protocol with EZ1 DNA Bacterial Card specifications or using QiaSymphony AS (Qiagen) as previously described.23 (link)
Qiasymphony as
Manufactured by Qiagen
The QiaSymphony AS is a fully automated sample preparation system designed for high-throughput nucleic acid purification. It offers efficient and reliable processing of a wide range of sample types, providing consistent results with minimal hands-on time.
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Lab products found in correlation
3 protocols using qiasymphony as
1
Bacterial DNA Extraction Using EZ1 and QiaSymphony
2
Bacterial DNA Extraction Using EZ1 and QiaSymphony
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Bacterial DNA was extracted using BioRobot EZ1 following the EZ1 DNA Tissue Kit protocol with EZ1 DNA Bacterial Card specifications or using QiaSymphony AS (Qiagen) as previously described.23 (link)
3
Comparison of CMV PCR Extraction Methods
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For the reference method, 100 µl of each specimen was extracted on the MagNA Pure LC (Roche, Indianapolis, IN) using the MagNA Pure LC DNA isolation kit with the DNA I Blood Cell High Performance protocol. The CACM assay was set-up manually using 50 µl out of the 100 µl elution.
For the test methods, 400 µl of specimen was extracted on the QIAsymphony SP (Qiagen, Germantown, MD) using the Qiagen Virus/Bacteria Midi kit and Pathogen Complex 400 protocol. Specimens were also extracted using the same kit and the Cell-free 1000 protocol, which extracts from 1000 µl of sample. For eight negative tissue specimens, there was insufficient volume for extraction using the Cell-free 1000 protocol. The artus CMV PCR assay (Qiagen, Germantown, MD) was set-up with a custom protocol on the QIAsymphony AS that utilizes 10 µl of the 95 µl elution, 12.5 µl CMV RG Master, and 2.5 µl of CMV Mg-Sol, for a final reaction volume of 25 µl.
For the test methods, 400 µl of specimen was extracted on the QIAsymphony SP (Qiagen, Germantown, MD) using the Qiagen Virus/Bacteria Midi kit and Pathogen Complex 400 protocol. Specimens were also extracted using the same kit and the Cell-free 1000 protocol, which extracts from 1000 µl of sample. For eight negative tissue specimens, there was insufficient volume for extraction using the Cell-free 1000 protocol. The artus CMV PCR assay (Qiagen, Germantown, MD) was set-up with a custom protocol on the QIAsymphony AS that utilizes 10 µl of the 95 µl elution, 12.5 µl CMV RG Master, and 2.5 µl of CMV Mg-Sol, for a final reaction volume of 25 µl.
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