Prepared sample was analysed by LCQ mass spectrometry by NICEM at Seoul National University. An Acquity HSS T3 column with 1.8 μm particles with Solvent A (Water, 0.2% formic acid) and solvent B (98% acetonitrile, 2% water with 0.2% formic acid) were used. Separation was carried out under the following condition: 5 min 90% A, 2.5 min 90–0% A, 10 min 0% A. then a fast return in 0.1 min to 100% A and 2 min for column equilibration. The flow rate was increased from 350 μL/min. Mass spectrometry was carried out with a Thermo Finnigan LCQ Deca XP Plus mass spectrometer operated in positive and negative mode. A full scan (m/z 50–2000) at 30,000 and 60,000 resolving power was conducted. Ions were generated through probe electrospray ionization (PESI). For collision induced dissociation (CID), normalized collision energy of 25% was used.
Lcq deca xp plus mass spectrometer
The LCQ Deca XP Plus mass spectrometer is a high-performance liquid chromatography-mass spectrometry (LC-MS) instrument designed for qualitative and quantitative analysis of a wide range of analytes. It utilizes ion trap technology to provide accurate mass measurements and high sensitivity detection.
Lab products found in correlation
7 protocols using lcq deca xp plus mass spectrometer
Comparative Metabolomics of Insect Herbivores
Prepared sample was analysed by LCQ mass spectrometry by NICEM at Seoul National University. An Acquity HSS T3 column with 1.8 μm particles with Solvent A (Water, 0.2% formic acid) and solvent B (98% acetonitrile, 2% water with 0.2% formic acid) were used. Separation was carried out under the following condition: 5 min 90% A, 2.5 min 90–0% A, 10 min 0% A. then a fast return in 0.1 min to 100% A and 2 min for column equilibration. The flow rate was increased from 350 μL/min. Mass spectrometry was carried out with a Thermo Finnigan LCQ Deca XP Plus mass spectrometer operated in positive and negative mode. A full scan (m/z 50–2000) at 30,000 and 60,000 resolving power was conducted. Ions were generated through probe electrospray ionization (PESI). For collision induced dissociation (CID), normalized collision energy of 25% was used.
Enzymatic Sulfation of 5α-Cyprinol
Quantification of Thiol Precursors in Grape Juice
Analysis was performed on a Thermo Finnigan Surveyor HPLC fitted with an Alltima C18 HPLC column (250 × 2.1 mm i.d., 5 µm, 100 Å, Grace Davison Discovery Sciences, Rowville, VIC, Australia) connected to a Thermo Finnigan LCQ Deca XP Plus mass spectrometer using electrospray ionisation in positive ion mode. Chromatographic conditions and ion pairs were as described previously (Capone, Sefton, Hayasaka, & Jeffery, 2010) and helium was used as collision gas with the following source and mass spectrometer conditions: spray voltage of 4.5 kV, respective sheath and aux/sweep gas flow rates of 30 and 19, capillary voltage of 36 V, capillary temperature of 250 °C, single reaction monitoring mode with activation Q of 0.250, activation time of 30 ms, normalised collision energy of 35%, and isolation width m/z = 1.50. Xcalibur software (Thermo Finnigan, version 1.3) was used for instrument control and data acquisition. Cys-3-SH and Glut-3-SH concentrations were reported as the sum of the two respective diastereomers.
Analytical Characterization of Compounds
Low-resolution HPLC-ESI-MS measurements were carried out by a Surveyor HPLC system (Thermo Fisher, San Jose, CA, USA) connected by a T splitter to a photodiode-array detector and an LCQ Deca XP Plus mass spectrometer (Thermo Fisher). The mass spectrometer was equipped with an ESI source. High-resolution HPLC-ESI-MS/MS experiments were carried out by an Ultimate 3000 Micro HPLC apparatus (Dionex, Sunnyvale, CA, USA) equipped with a FLM-3000-Flow manager module and coupled to an LTQ Orbitrap XL apparatus (Thermo Fisher) (12 (link)).
Protein N- and C-terminal Sequencing
Analytical Instrumentation for Multidisciplinary Research
Radiolabeling of NOTA-Conjugated Oligonucleotides
Aqueous triethylammonium acetate (TEAA) (1 M, pH 7.0) was prepared with 1 M acetic acid and 1 M TE (Sigma-Aldrich). A Thermo LCQ Deca XP plus Mass Spectrometer was used to record ESI-MS spectra.
Products were isolated and puri ed using a HPLC C18 column (1200 series, Zorbax ODS 4.6 × 250 mm). HPLC conditions were as follows: A/B gradient; A: 2% acetonitrile in 0.1 M TEAA (pH 7.0); B: 50% acetonitrile in 0.1 M TEAA (pH 7.0); elution: 1 mL/min. A 68 Ge-68 Ga generator (ITG Isotope technologies) was used to produce [ 68 Ga] Cl 3 .
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