The dual-luciferase reporter gene plasmids containing wild-type and mutant sequences were synthesized using Promega (Madison, WI, USA). The wild-type or mutant (NEAT1, SERPINE1) reporter plasmid and miR-10a-5p mimic or mimic-NC were co-transfected into 293T cells. Luciferase activity was measured 48 h after transfection.
Dual luciferase reporter gene plasmids
Manufactured by Promega
Sourced in United States
Dual-luciferase reporter gene plasmids are genetic constructs that contain two distinct luciferase reporter genes, typically firefly and Renilla luciferase. These plasmids enable the simultaneous measurement of two distinct reporter activities within the same experimental system.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dual luciferase reporter gene plasmids
1
NEAT1 and SERPINE1 Luciferase Assay
2
Luciferase Assay for miRNA Targeting
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The wild-type and mutant sequences were synthesized and cloned into dual-luciferase reporter gene plasmids (Promega) containing the psiCheck2 promoter to biologically predict the possible miRNA target genes and binding sequences. After SiHa cells were inoculated into a 96-well plate and cultured for 24 h, the cells were co-transfected with wild-type or mutant reporter gene plasmids and overexpression or silencing plasmid mimics. Luciferase activity was measured 48 h after transfection.
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