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Nr3c1 is a protein that functions as a nuclear receptor. It is involved in the regulation of gene expression and plays a role in various physiological processes. The detailed function and applications of this product are not available at this time.

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Lab products found in correlation

Universal probes one step pcr kit, by Bio-Rad (1 mentions) Wfdc2, by Thermo Fisher Scientific (1 mentions) Cfx96 real time pcr system, by Bio-Rad (1 mentions) Taqman gene expression kit, by Thermo Fisher Scientific (1 mentions) Fstl1, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Steponeplus, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions)

2 protocols using nr3c1

1

qPCR Expression Profiling of Mouse Genes

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Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the Taqman primers Cftr (Mm00445197_m1), Olfm4 (Mm01320260_m1), Wfdc2 (Mm00509434_m1), Il13 (Mm00434204_m1), Csf2 (Mm01290062_m1), Ar (Mm00442688_m1), and Nr3c1 (Mm00433832_m1) (ThermoFisher) on a CFX96 Real Time PCR system (Bio-Rad Laboratories). Messenger RNA levels were normalized to the reference gene Ppib (Mm00478295_m1).
Busada J.T., Peterson K.N., Khadka S., Xu X., Oakley R.H., Cook D.N, & Cidlowski J.A. (2021). Glucocorticoids and androgens protect from gastric metaplasia by suppressing group 2 innate lymphoid cell activation.. Gastroenterology, 161(2), 637-652.e4.
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2

Quantitative Gene Expression Analysis

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After treatment, cells were harvested and total RNA was extracted via the RNeasy Mini Kit (Qiagen, Valencia, CA) and reverse transcribed with a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). The TaqMan Gene Expression kit (Life Technologies) and StepOne Plus (Applied Biosystems) were employed for quantitative analyses. Taqman primer probes for ACTA2 (Hs00426835_g1), SERPINE1 (Hs00167155_m1), COL1A1 (Hs00164004_m1), FN1 (Hs01549976_m1), CCN2 (Hs1026927_g1), EDN1 (Hs00174961_m1), FSTL1 (Hs00907496_m1), TSC22D3 (Hs00608272_m1), NR3C1 (Hs00353740_m1), YAP1 (Hs00902712_g1), and GAPDH (Hs02758991_g1) were employed (all from Thermo Fisher Scientific, Waltham, MA). The ΔΔCt method was employed with GAPDH as the housekeeping gene for quantification of relative expression.
Nakamura R., Bing R., Doyle C.P., Garabedian M.J, & Branski R.C. (2021). Glucocorticoids activate Yes-associated protein in human vocal fold fibroblasts. Experimental cell research, 405(2), 112681.
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