Cultured cells were lysed with lysis buffer. Equal amounts of protein were run on 10% SDS-PAGE and electrotransferred to polyvinylidene fluoride membranes (Millipore). After blocking with 5% milk in TBST, membranes were incubated with primary antibodies overnight. The following antibodies were used: anti-HPVE6 (1:1000, Arigo), anti-HPVE7(1:1000, Bioss), (anti-CXCL10 (1:1,000, Abcam), anti-CXCR3 (1:1,000, Boster), anti-PDL1 (1:2,000, proteintech), anti-STAT1 (1:1,000, proteintech), anti-pSTAT1 (1:1,000, Abcam), anti-JAK1 (1:2,000, proteintech), anti-GAPDH (1:6,000, Yeasen) and anti-tublin (1:3,000, Yeasen). Membranes were then incubated with the rabbit peroxidase-conjugated secondary antibody (1:10,000, Abclonal). The blots were detected by sensitive chemiluminescence liquid analysis (Yeasen) and Biorad software was used to capture the images.
Anti tublin
Manufactured by Yeasen
Anti-tublin is a lab equipment product that functions as an inhibitor of tubulin polymerization. It is designed to disrupt the assembly of microtubules, which are essential cellular structures involved in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Anti stat1, by Proteintech (1 mentions)
Anti gapdh, by Yeasen (1 mentions)
Anti p stat1, by Abcam (1 mentions)
Anti jak1, by Proteintech (1 mentions)
Anti cxcl10, by Abcam (1 mentions)
Rabbit peroxidase conjugated secondary antibody, by ABclonal (1 mentions)
Anti pd l1, by Proteintech (1 mentions)
Ham s f12 medium, by Thermo Fisher Scientific (1 mentions)
Anti wdr5, by Abcam (1 mentions)
Anti actin, by Merck Group (1 mentions)
Anti flag tag, by Affinity Biosciences (1 mentions)
Anti histone h3k4me3q5ser, by Merck Group (1 mentions)
Anti histone h3, by Immunoway (1 mentions)
2 protocols using anti tublin
1
Western Blot Profiling of HPV Biomarkers
2
Characterization of Human Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human neuroblastoma cell line SK-N-SH and the human embryonic kidney cell line (HEK 293T) were obtained from the Cell Bank of Chinese Academy of Sciences. The SK-N-SH and HEK 293T cells were cultured in 1:1 Eagle's Minimum Essential Medium (EMEM) (ATCC) and Ham's F12 medium (Thermo) supplemented with 15% fetal bovine serum (FBS) and Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% FBS, and incubated at 37°C in a humidified incubator with 5% CO2.
The antibodies used in current study included anti-WDR5 (Abcam, ab178410); anti-Histone H3K4me3Q5ser (Millipore, ABE2580); anti-FLAG tag (Affinity, T0003); anti-Histone H3 (Immunoway, YM3038); anti--Actin (Sigma, A3854); anti--Tublin (Yeasen, 30303ES50); HRP-conjugated anti-rabbit IgG (CST, 7074S); and HRP-conjugated antimouse IgG (CST, 7076S).
The antibodies used in current study included anti-WDR5 (Abcam, ab178410); anti-Histone H3K4me3Q5ser (Millipore, ABE2580); anti-FLAG tag (Affinity, T0003); anti-Histone H3 (Immunoway, YM3038); anti--Actin (Sigma, A3854); anti--Tublin (Yeasen, 30303ES50); HRP-conjugated anti-rabbit IgG (CST, 7074S); and HRP-conjugated antimouse IgG (CST, 7076S).
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