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5 protocols using sodium hydrogen carbonate

1

Adjuvant-Induced Inflammation Model

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Complete Freund's Adjuvant (CFA) (10 mL; each 1 mL of CFA contains 1 mg of Mycobacterium tuberculosis, heat-killed and dried, 0.85 mL paraffin oil, and 0.15 mL mannide monooleate) was obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Curcumin was obtained from Hedel–De Han AG, Germany. DMEM (Dulbecco's modified Eagle's medium), trypsin/EDTA, penicillin-streptomycin solution, and fetal bovine serum (FBS) were obtained from Lonza, Belgium. Sodium hydrogen carbonate was obtained from LOBA Chemie, India. Culture flasks and culture consumables were obtained from Greiner Bio-One (Germany). Other reagents and all chemicals used were of analytical quality and high purity.
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2

Investigating the Effects of STZ and Hesperetin

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Both STZ and hesperetin were acquired from Sigma Chemical Co. (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), penicillin/streptomycin solution, trypsin/ethylene diamine tetra acetic acid (EDTA), and fetal bovine serum (FBS) were obtained from Lonza, Antwerp, Belgium. Sodium hydrogen carbonate was obtained from LOBA Chemie, Mumbai, Maharashtra, India. Culture flasks and culture consumables were obtained from Greiner Bio-One (Frickenhausen, Germany). All other reagents utilized in the experiments were of analytical quality and highly pure.
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3

Cell Culture Media and Reagents

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Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin solution, and trypsin/EDTA were obtained from Lonza, Belgium. Sodium hydrogen carbonate was purchased from LOBA Chemie, India. Culture consumables and culture flasks were purchased from Greiner Bio-One (Germany).
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4

Porphyrin Synthesis and Characterization

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Pyrrole, 4-bromobenzaldehyde, dichloromethane (DCM), methanol (MeOH), high purity grade silica gel (35–60 mesh particle size, thin layer chromatography (TLC) plates, sodium acetate, 5-bromo-2-thiophenecarboxaldehyde, indium(III) chloride, tetraphenylporphyrin (H2TPP), zinc tetraphenylporphyrin (ZnTPP) and gallium (III) chloride were purchased from Sigma Aldrich (Munich, Germany). Hexane, propionic acid, sodium hydrogen carbonate, acetic acid and zinc acetate were purchased from Loba Chemie PVT Ltd, Mumbai, India. Toluene and ethyl acetate were purchased from Fisher Scientific, Leicestershire, UK. Distilled water was obtained from an ultrapure filtration system (Millipore, resistivity 18 mΩ cm−1). TiO2 P25 was purchased from Univar Canada.
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5

Formulation and Characterization of Pantoprazole Sodium Enteric Coated Tablets

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Pantoprazole Sodium procured from Marksan Pharmaceutical Ltd., Goa, Sodium Benzoate from Central Drug House, New Delhi, Sodium Hydrogen Carbonate from Loba Chemie Pvt Ltd, Mumbai, Gallic Acid from Labogens Fine Chem Ind. New Delhi, Iso propyl alcohol, Methanol from Fisher Scientific India Pvt. Ltd. New Delhi, Potassium Dihydrogen orthophosphate and Disodium hydrogen orthophosphate from Thomas Baker, New Delhi were obtained.
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