1200 series binary pump g1312b

Melting points were determined on a capillary tube melting point apparatus equipped with a digital thermometer. NMR spectra were recorded in DMSO-d6 on a Bruker NMR spectrometer operating at 500 MHz for 1H (with TMS as an internal standard) and 125 MHz for 13C. were performed on reverse phase gradient using Agilent (Santa Clara, CA, USA) 1200 series binary pump (G1312B), waters XTerra MS C18 (3.5 mm; 2.1–150 mm) þ Phenomenex C18 security guard column (2–4 mm) using 0.2% acetic acid in H2O/methanol as mobile phases; wavelength ¼ 254 nm; and mass spectrometry was done with 6220 Agilent (Santa Clara, CA, USA) TOF in electrospray ionization (ESI) mode with a positive and negative method in both Profile and Centroid mode. HPLC studies were done with 6120 Agilent (quadrupole LC/MS) [29 (link)].

All materials were purchased from commercial suppliers and used with no extra purification. The final compounds' purities were elucidated by tandem mass spectrometry (LC/MS) using a gradient elution system (acetonitrile/water 5/95/95/5, 5 min, 0.05% formic acid) on Ascentis Express Peptide C18 column, and UV detection (254 nm). The final compounds' purities were 95% or greater. A Bruker NMR 400 MHz Avance III spectrometer operating at 100 MHz for ¹³C NMR and 400 MHz for ¹H NMR was utilised for NMR spectra recording. Chemical shifts are given relative to tetramethylsilane (TMS) in part per million (ppm), and coupling constants J are given in Hertz. HPLC-HRMS analyses were carried out using Agilent (Santa Clara, CA) 1200 series binary pump (G1312B), and columns waters XTerra MS C₁₈ (3.5 um; 2.1 × 150 mm) + Phenomenex C₁₈ security guard column (2 × 4 mm) on gradient elution mobile phase using 0.2% acetic acid in H₂O/methanol; wavelength = 254 nm. Elemental analyses were established at Microanalytical Centre, Faculty of Science, Cairo University, Egypt using Manual Elemental Analyser Heraeus (Germany) and Automatic Elemental Analyser CHN Model 2400 Perkin Elmer (USA).