Mouse monoclonal antibodies against actin

Western blot analysis was performed as described before [26 (link)]. Briefly, the mock or virus infected cells were collected at different time points after infection, washed with PBS and processed with RIPA lysis buffer. Equal amounts of total proteins were used for Western blot analysis. The membranes were first probed with primary antibody, washed, incubated with secondary antibody, again washed and detected using the chemiluminescence substrate and exposure to X-ray film. The detection antibodies were as follows: rabbit antibodies against phospho-STAT1 and rabbit polyclonal antibodies against STAT1 (Cell Signaling Technology, Danvers, MA, USA); mouse monoclonal antibodies against actin (Ambion, Thermo Fisher Scientific); mouse monoclonal antibodies against PKR (Santa Cruz Biotechnology); rabbit antibodies against ISG15 (Cell Signaling Technology). Generation of rabbit polyclonal and mouse monoclonal antibodies against MYXV proteins M-T7 and Serp-1 was described before [32 (link),33 (link)].

Western blot analysis was performed as described before¹⁰ . Briefly, the mock or siRNA transfected cells were collected at different time points after transfection, washed with PBS and processed with RIPA lysis buffer. Equal amount of total proteins were used for Western blot analysis. The membranes were first probed with primary antibody, washed, incubated with secondary antibody, washed again and detected using the chemiluminescence substrate and exposure to X-ray film. The detection antibodies were as follows: mouse monoclonal antibodies against DHX9 and DHX29 (Santa Cruz Biotechnology); rabbit polyclonal antibodies against DHX37 (abcam, USA) and DHX35 (Novus Biologicals, USA); mouse monoclonal antibodies against actin (Ambion, Thermo Fisher Scientific).