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2 protocols using donkey anti goat680

1

Cytoskeletal Modulator Compound Characterization

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Vemurafenib (#S1267), Y-27632 (#S1049), fasudil (#S1573), and dasatinib (#S1021) were purchased from Sellekchem, Houston, TX, USA. Latrunculin B (#10010631), cytochalasin D (#11330), and erlotinib (#10483) were purchased from Cayman Chemical, Ann Arbor, MI, USA. Rho Inhibitor I (#CT04-A) was purchased from Cyoskeleton Inc, Denver, CO, USA. CCG-222740 18 (link) was synthesized in the lab of Dr. Scott Larsen at the University of Michigan. All compounds were diluted in DMSO to a stock concentration of 10 mM. Compound stock solutions were frozen at −20 °C. Antibodies against YAP1 (#14074), MLC2 (#3672), pMLC2 (#3674), Sox10 (#89356), and pEGFR (#3777) were purchased from Cell Signaling, Danvers, MA, USA. Antibodies against MRTF-A (#sc21558), MRTF-B (#sc98989), and Actin (#sc1616) were purchased from Santa Cruz, Dallas, TX, USA. Donkey anti-Mouse800 (#926-32212), Donkey anti-Goat680 (#926-68074), and Donkey anti-Rabbit680 (#926-68073) immunoblotting secondary antibodies were purchased from LI-COR, Lincoln, NE, USA. Alexa Fluor goat anti-rabbit488 (#A11034) and donkey anti-goat488 (#A11055) were purchased from Invitrogen. Alexa Fluor546 Phalloidin (#A22263) was purchased from ThermoFisher.
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2

Cytoskeletal Modulator Compound Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vemurafenib (#S1267), Y-27632 (#S1049), fasudil (#S1573), and dasatinib (#S1021) were purchased from Sellekchem, Houston, TX, USA. Latrunculin B (#10010631), cytochalasin D (#11330), and erlotinib (#10483) were purchased from Cayman Chemical, Ann Arbor, MI, USA. Rho Inhibitor I (#CT04-A) was purchased from Cyoskeleton Inc, Denver, CO, USA. CCG-222740 18 (link) was synthesized in the lab of Dr. Scott Larsen at the University of Michigan. All compounds were diluted in DMSO to a stock concentration of 10 mM. Compound stock solutions were frozen at −20 °C. Antibodies against YAP1 (#14074), MLC2 (#3672), pMLC2 (#3674), Sox10 (#89356), and pEGFR (#3777) were purchased from Cell Signaling, Danvers, MA, USA. Antibodies against MRTF-A (#sc21558), MRTF-B (#sc98989), and Actin (#sc1616) were purchased from Santa Cruz, Dallas, TX, USA. Donkey anti-Mouse800 (#926-32212), Donkey anti-Goat680 (#926-68074), and Donkey anti-Rabbit680 (#926-68073) immunoblotting secondary antibodies were purchased from LI-COR, Lincoln, NE, USA. Alexa Fluor goat anti-rabbit488 (#A11034) and donkey anti-goat488 (#A11055) were purchased from Invitrogen. Alexa Fluor546 Phalloidin (#A22263) was purchased from ThermoFisher.
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