Log In Sign up
The largest database of trusted experimental protocols

Antibiotic antimycotic agent

Manufactured by Corning
Visit Supplier
Sourced in United States

Antibiotic–antimycotic agent is a laboratory product designed to inhibit the growth of bacteria, fungi, and other microorganisms. It is commonly used in cell culture and other laboratory procedures to prevent microbial contamination.

Automatically generated - may contain errors

Lab products found in correlation

Bovine serum, by Corning (1 mentions) Sonidegib, by Selleck Chemicals (1 mentions) Fluorescent mounting medium, by Agilent Technologies (1 mentions) Anti mouse and anti rabbit secondary antibodies, by Cell Signaling Technology (1 mentions) Anti β actin and anti lamin b1 antibodies, by Merck Group (1 mentions) Dulbecco s modified eagle medium dmem, by Corning (1 mentions) Fibroblast growth factor (fgf), by Merck Group (1 mentions) 24 well ultra low attachment plate, by Corning (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions)

Close spelling variants of this product

Antibiotic-antimycotic Antibiotics/Antimycotics

2 protocols using antibiotic antimycotic agent

1

Molecular Mechanisms of Anti-inflammatory Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following substances were used in this study: Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and antibiotic–antimycotic agent (Corning Life Science, Corning, NY, USA); sonidegib (Selleckchem, Houston, TX, USA); SP600125 (Calbiochem, Darmstadt, Germany); LPS from Escherichia coli (serotype O111:B4), dimethyl sulfoxide (DMSO), and anti-β-actin and anti-lamin B1 antibodies (Sigma-Aldrich, St. Louis, MO, USA); goat anti-mouse IgG secondary antibody conjugated with Alexa Fluor 488 and 4′,6-diamidino-2-henylindole dihydrochloride (DAPI; Thermo Scientific, Rockford, IL, USA); fluorescent mounting medium (Dako, Carpinteria, CA, USA); MKK4 antibody (Santa Cruz Biotechnology, Dallas, TX, USA); anti-phospho-IκBα antibody (Abcam, Cambridge, MA, USA); and primary antibodies against phospho-MKK4, phospho-JNK, JNK3, phospho-c-Jun, c-Jun, iNOS, COX-2, NF-κB p65, and IκBα and secondary anti-rabbit and anti-mouse antibodies (Cell Signaling Technology, Danvers, MA, USA).
Nguyen N.M., Duong M.T., Bui B.P., Nguyen P.L., Chen X., Cho J, & Ahn H.C. (2022). Sonidegib Suppresses Production of Inflammatory Mediators and Cell Migration in BV2 Microglial Cells and Mice Treated with Lipopolysaccharide via JNK and NF-κB Inhibition. International Journal of Molecular Sciences, 23(18), 10590.
+ Open protocol
+ Expand
2

Compound 11 Inhibits Colon Cancer Colonosphere Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols
In order to examine the ability of compound 11 to inhibit colonosphere formation of colon cancer cell lines, HCT-116 cells were harvested and diluted as single-cell suspensions. Approximately 2,500 cells were added into each well of 24-well in ultralow-binding plate, with or without compound 11. The cells were maintained in serum-free Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12, Gibco) supplemented with B27 (Life Technologies), 20 ng/ml epidermal growth factor (Sigma), 10 ng/ml fibroblast growth factor (Sigma), and antibiotic–antimycotic agent in 24-well ultralow attachment plates (Corning Inc). Fresh aliquots of the growth factors were added to the culture medium every other day. The cells were cultured for 10 days, and the effect of compound 11 on colonospheres was observed.
Lagisetty P., Eeda V., Yadav V.R., Nimmo S.L., Subramaniam D., Powell D.R, & Awasthi V. (2020). Tumor suppressive activities of solvatochromic 3,3′-azadimethylene dinaphthospiropyran in colon cancer model. Chemical biology & drug design, 97(2), 325-340.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!