The kidney was set in 10% paraformaldehyde, dehydrated, fixed in paraffin, and sectioned at 5 μm. Hematoxylin and eosin were used to stain the sections. The morphological changes were examined under a microscope (Eclipse 80i, Nikon, Japan), and the pictures were captured with a video camera (DS-Fi1 digital microscope camera, Nikon, Japan).
Ds fi1 digital microscope camera
Manufactured by Nikon
Sourced in Japan
The DS-Fi1 is a digital microscope camera from Nikon. It is designed to capture high-quality images from microscopes. The camera features a 5 megapixel sensor and supports various output resolutions.
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4 protocols using ds fi1 digital microscope camera
1
Kidney Histology Preparation and Analysis
2
Histological Analysis of Kidney Tissue
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The kidney tissue sections were dehydrated in ethanol, cleared in xylol, fixed in a buffered formaldehyde solution (10%) for 24 h, infiltrated with paraffin wax for another hour, and then embedded in paraffin wax. The 5 μm thick paraffin slices were stained with hematoxylin and eosin (H&E). A microscope (Eclipse 80i, Nikon, Japan) was employed to assess the morphological alterations, and video cameras (DS-Fi1 digital microscope camera, Nikon) were utilized to capture the pictures.
3
Quantifying Cell Morphology Dynamics
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Bright field phase contrast images of cells cultured in complete medium were taken at equal confluence and at different time points in a Nikon Eclipse TE300 Inverted microscope equipped with a DS-Fi1 digital microscope camera and a DS-U2 camera control unit (Nikon, Tokyo, Japan ). Ratios of cell length to cell width were acquired for all cells using the Straight line tool in Image J 1.48 program. A minimum of 200 cells was randomly measured per time point and ratio medians calculated.
4
Histological Analysis of Rat Testes
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The right testes were fixed in 10% paraformaldehyde, dehydrated and embedded in paraffin, sectioned at 5 μm. The sections were stained with haematoxylin and eosin. The morphological changes were examined under a microscope (Eclipse 80i, Nikon, Japan), and the pictures were captured with a video camera (DS-Fi1 digital microscope camera, Nikon, Japan).
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