Blood was obtained by via cardiac puncture using a 10mL heparinized syringe. Three hundred microliter aliquots were then sampled and analyzed within 10 minutes of collection using a Coulter Hmx Hematology Analyzer (Beckman Coulter; Brea, CA).
Coulter hmx hematology analyzer
Manufactured by Beckman Coulter
Sourced in United States
The Coulter HmX Hematology Analyzer is a diagnostic instrument designed for the automated analysis of blood samples. It provides complete blood count (CBC) and differential results. The analyzer uses the Coulter principle to count and size blood cells, determining various hematological parameters.
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Lab products found in correlation
4 protocols using coulter hmx hematology analyzer
1
Cardiac Puncture Blood Sampling
2
Comprehensive Hematological Analysis
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Hematological measurements and calculations were performed by using Coulter HmX Hematology Analyzer (Beckman Coulter Inc., Fullerton, CA, United States). The following twelve indicators were evaluated in hematology: RBC, HGB concentration, HCT, MCV, MCH, MCHC, RDW, PCT, blood PLT, MPV, PDW, and WBC.
3
Comprehensive Blood Analysis Protocol
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For the hematology examination, one part of blood samples was corrected and treated with ethylene diamine tetra-acetic acid (EDTA) to analyze the hematological parameters including red blood cell count (RBC), hemoglobin concentration (HG), blood platelet hematocrit (PLT), and white blood cell count (WBC) by a Coulter HmX Hematology Analyzer (Beckman Coulter Inc., Fullerton, CA, USA).
For the clinical chemistry parameter examination, another part of the blood sample was centrifuged at 3,000 g for 10 min and the supernatant was collected to examine clinical chemistry parameter by using a Synchron Clinical System CX4 (Beckman Coulter, Brea, CA, USA) according to the manufacturer's instructions (Beijing Leadman Biochemistry Technology Co. Ltd., Beijing, China). These parameters included alanine aspartate aminotransferase (AST), aminotransferase (ALT), protein (TP), albumin (Alb), total cholesterol (TCH), creatinine (Cr), and blood urea nitrogen (BUN).
For the clinical chemistry parameter examination, another part of the blood sample was centrifuged at 3,000 g for 10 min and the supernatant was collected to examine clinical chemistry parameter by using a Synchron Clinical System CX4 (Beckman Coulter, Brea, CA, USA) according to the manufacturer's instructions (Beijing Leadman Biochemistry Technology Co. Ltd., Beijing, China). These parameters included alanine aspartate aminotransferase (AST), aminotransferase (ALT), protein (TP), albumin (Alb), total cholesterol (TCH), creatinine (Cr), and blood urea nitrogen (BUN).
4
Framingham Heart Study Blood Profiling
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The Framingham Heart Study (FHS) is a population-based study, predominantly of European ancestry, consisting of an ongoing series of primarily family-based cohorts first developed in 1948 and based in Framingham, MA, USA; it comprises the Original 18 , Offspring 19 , and Third Generation 20 cohorts. FHS gene expression, blood cell counts, subject and sample metadata was obtained from dbGap (phs000007). Gene expression data was processed (filtered and normalized) as in 28 , resulting in 5,058 available FHS samples, of which 3,728 had available blood cell counts. Blood cell counts were obtained through a Complete Blood Count using the Coulter HmX hematology analyzer (Beckman Coulter, Inc.) 29, 30 .
The "gold standard" is cell counts and cell percent. The cell counting was performed on a Beckman Coulter HmX hematology analyzer. The following metrics from whole blood were obtained -HbA1c, basophil count and percent, eosinophil count and percent, hematocrit, hemoglobin, lymphocyte count and percent, MCH, MCHC, MCV, monocyte count and percent, MPV, neutrophil count and percent, platelet count, RBC, RDW, and WBC. Further information is available here: https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/GetPdf.cgi?id=phd004086.1.
The "gold standard" is cell counts and cell percent. The cell counting was performed on a Beckman Coulter HmX hematology analyzer. The following metrics from whole blood were obtained -HbA1c, basophil count and percent, eosinophil count and percent, hematocrit, hemoglobin, lymphocyte count and percent, MCH, MCHC, MCV, monocyte count and percent, MPV, neutrophil count and percent, platelet count, RBC, RDW, and WBC. Further information is available here: https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/GetPdf.cgi?id=phd004086.1.
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