Normal human dermal fibroblasts (HFIB) were acquired from PromoCell (Labclinics, S.A., Barcelona, Spain). T-flasks, cell culture plates and other cell culture consumables were obtained from ThermoFisher Scientific (Porto, Portugal). Cell imaging plates were bought from Ibidi GmbH (Ibidi, Munich, Germany). Fetal bovine serum (FBS) was purchased from Biochrom AG (Berlin, Germany). PI was procured from Invitrogen (Carlsbad, CA, USA). Agarose was obtained from Grisp (Porto, Portugal). Dulbecco’s modified Eagle’s medium F12 (DMEM-F12), paraformaldehyde (PFA), phosphate-buffered saline (PBS), trypsin, formamide (≥99.5%), ethylenediaminetetraacetate (EDTA), and PEG 4000 Da were purchased from Sigma-Aldrich (Sintra, Portugal).
Cell imaging plates
Manufactured by Ibidi
Sourced in Germany
Cell imaging plates are a type of laboratory equipment designed for cell culture and microscopic observation. They provide a standardized platform for seeding, culturing, and imaging cells in a controlled environment. These plates typically feature a flat, transparent surface and multiple wells to accommodate multiple cell samples simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Harvard Bioscience (5 mentions)
Cell culture plates, by Thermo Fisher Scientific (3 mentions)
T flasks, by Thermo Fisher Scientific (3 mentions)
Paraformaldehyde (pfa), by Merck Group (3 mentions)
Normal human dermal fibroblasts hfib, by PromoCell (2 mentions)
Calcein am, by Thermo Fisher Scientific (2 mentions)
3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium mts, by Promega (2 mentions)
Propidium iodide, by Thermo Fisher Scientific (2 mentions)
Trypsin, by Merck Group (2 mentions)
Formamide 99.5, by Merck Group (1 mentions)
Ethylenediaminetetraacetate edta, by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Merck Group (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Mcf 7 cell line, by American Type Culture Collection (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Cell culture plates and t flasks, by Thermo Fisher Scientific (1 mentions)
Ir 780 iodide, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium f12 dmem f12, by Merck Group (1 mentions)
5 protocols using cell imaging plates
1
Culturing Normal Human Dermal Fibroblasts
2
Cytotoxicity Evaluation of Nanomaterials
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Michigan Cancer Foundation-7 (MCF-7) cell line and Normal Human Dermal Fibroblasts (NHDF) were obtained from ATCC (Middlesex, UK) and Promocell (Heidelberg, Germany), respectively. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) was bought from Promega (Madison, WI, USA). Fetal Bovine Serum (FBS) was acquired from Biochrom AG (Berlin, Germany). 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was purchased from Merck (Darmstadt, Germany). Dimethyl sulfoxide (DMSO) and methanol were acquired from Fisher Scientific (Oeiras, Portugal). Cell imaging plates were obtained from Ibidi GmbH (Munich, Germany). Cell culture plates and T-flasks were purchased from Thermo Fisher Scientific (Porto, Portugal). GO was obtained from NanoPoz (Umultowska Poznan, Wielkopolska). Branched PEI (Mn 1250 Da), SBMA, Dulbecco's Modified Eagle's Medium-F12 (DMEM-F12), IR780 iodide, N-hydroxysuccinimide (NHS), paraformaldehyde, PMAO (average Mn 30 000–50 000 Da) and trypsin were bought from Sigma-Aldrich (Sintra, Portugal). Calcein-AM, Hoechst 33342® and Propidium Iodide (PI) were obtained from Thermo Fisher Scientific (Porto, Portugal). Water used in all experiments was double deionized (0.22 μm filtered, 18.2 MΩ cm).
3
Cytotoxicity Assay of Graphene Oxide
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DMEM-F12, IR780, DL-Dithiothreitol (DTT) and SBMA were purchased from Sigma-Aldrich (Sintra, Portugal). Methanol was obtained from Honeywell (Oeiras, Portugal). BSA was obtained from Amresco (PA, USA). 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) was bought from Promega (WI, USA). Michigan Cancer Foundation-7 (MCF-7) cell line was acquired from ATCC (Middlesex, UK). Normal human dermal fibroblasts (NHDF) were obtained from PromoCell (Heidelberg, Germany). Fetal bovine serum (FBS) was bought from Biochrom AG (Berlin, Germany). Calcein-AM, cell culture plates, T-flasks and propidium iodide (PI) were purchased from Thermo Fisher Scientific (Porto, Portugal). Cell imaging plates were obtained from Ibidi GmbH (Munich, Germany). GO was acquired from NanoPoz (Umultowska Poznan, Wielkopolska). SBMA-g-BSA was synthesized as previously described [48] . Water used in all assays was double deionized (0.22 μm filtered; 18.2 M cm).
4
Analyzing Breast Cancer Cell Response to Doxorubicin
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Estrogen-dependent human breast adenocarcinoma (MCF-7) cells were acquired from ATCC (Middlesex, UK). Cell culture plates and T-flasks were obtained from Thermo Fisher Scientific (Porto, Portugal). 2',7'-dichlorofluorescein diacetate (H 2 DCFDA), Dulbecco's Modified Eagle's medium F-12 (DMEM-F12), ethylenediaminetetraacetic acid (EDTA), gentamicin, L-glutathione (GSH), paraformaldehyde (PFA), phosphate-buffered saline solution (PBS), resazurin, rhodamine 123 (Rho 123), streptomycin, and trypsin were bought from Sigma-Aldrich (Sintra, Portugal). Agarose was obtained from Grisp (Porto, Portugal). Fetal bovine serum (FBS) was purchased from Biochrom AG (Berlin, Germany). DOX was obtained from Carbosynth (Berkshire, UK). Cell imaging plates were acquired from Ibidi GmbH (Ibidi). The store solution of DOX was prepared in Methanol gotten from VWR International (Portugal).
5
Culturing and Imaging Human Dermal Fibroblasts
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Normal human dermal fibroblasts (HFIB) were bought from PromoCell (Labclinics, S.A., Barcelona, Spain). Cell imaging plates were acquired from Ibidi GmbH (Ibidi, Munich, Germany). Agarose was purchased from Grisp (Porto, Portugal). Cell culture plates, T-flasks, and cell culture consumables were obtained from ThermoFisher Scientific (Porto, Portugal). Dulbecco's modified Eagle's medium F12 (DMEM-F12), formamide (≥99.5%), paraformaldehyde (PFA), phosphatebuffered saline (PBS), trypsin, ethylenediaminetetraacetate (EDTA), PEG 4000, 8000, and 10,000 Da were purchased from Sigma-Aldrich (Sintra, Portugal). Fetal bovine serum (FBS) was obtained from Biochrom AG (Berlin, Germany). PI was purchased from Invitrogen (Carlsbad, California).
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