Dextran 568

Manufactured by Thermo Fisher Scientific

Dextran-568 is a fluorescently labeled carbohydrate polymer that can be used as a molecular weight marker or tracer in various research applications. It has an absorption maximum at 568 nm and an emission maximum at 588 nm, making it suitable for detection using standard rhodamine or Texas Red filter sets.

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Lab products found in correlation

Szx16 dissecting microscope, by Olympus (1 mentions)

2 protocols using dextran 568

Dextran-568 Labeling of Drosophila Brains

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2.5 mM 10 kDa Dextran-568 (Thermo Fisher Scientific) was diluted 1:100 and applied to dissected L3 filets for 30 min at 25°C. Brain lobes were imaged following 3× 5 min washes in PBS.

McLaughlin C.N., Perry-Richardson J.J., Coutinho-Budd J.C, & Broihier H.T. (2019). Dying neurons utilize innate immune signaling to prime glia for phagocytosis during development. Developmental cell, 48(4), 506-522.e6.

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Lymph Node Dextran Accumulation

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50ul of 5 mg/ml dextran-568 (10 000 kD MW, ThermoFisher) was administered to anesthetized, intubated mice via endotracheal catheter. Fifty minutes after administration, the mice were sacrificed for procurement of mediastinal lymph nodes. Lymph nodes were imaged using an Olympus SZX16 dissecting microscope. Quantification of fluorescence intensity was performed using ImageJ.

Reed H.O., Wang L., Kahn M.L, & Hancock W.W. (2020). Donor-Host Lymphatic Anastomosis after Murine Lung Transplantation. Transplantation, 104(3), 511-515.

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