Metformin was obtained from Enzo Life Sciences (Farmingdale, NY). Primary antibodies for AMPKα1/α2 and pAMPKα1/α2 were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for LKB1, pLKB1 and β-actin were obtained from Santa Cruz Biotechnology (Dallas, TX). Primary antibody for PGC1-α was obtained from Abcam (Cambridge, MA). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800 and goat anti-mouse IR-680 antibodies, and the 4× Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The Multiplex Luminex Array kit, the NuPAGE® LDS Sample Buffer (4×), and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Primary antibodies for lkb1
Manufactured by Santa Cruz Biotechnology
The Primary antibodies for LKB1 are laboratory reagents designed to detect and study the Liver Kinase B1 (LKB1) protein in biological samples. LKB1 is a serine/threonine protein kinase that plays a crucial role in the regulation of cellular energy metabolism and other cellular processes. These primary antibodies can be used in various research applications, such as Western blotting, immunohistochemistry, and immunoprecipitation, to identify and quantify the expression of LKB1 in different cell types or tissues.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710, by Zeiss (2 mentions)
Nupage lds sample buffer 4, by Thermo Fisher Scientific (1 mentions)
Protein sample loading buffer, by LI COR (1 mentions)
Primary antibodies for ampkα1 α2, by Cell Signaling Technology (1 mentions)
Pampkα1 α2, by Cell Signaling Technology (1 mentions)
Goat anti rabbit ir 800 and goat anti mouse ir 680 antibodies, by LI COR (1 mentions)
Western blot gels, by Thermo Fisher Scientific (1 mentions)
Odyssey blocking buffer, by LI COR (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Metformin, by Enzo Life Sciences (1 mentions)
2 protocols using primary antibodies for lkb1
1
Metformin Regulation of AMPK Signaling
2
NRCM LKB1 Activation by CTRP9
Check if the same lab product or an alternative is used in the 5 most similar protocols
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NRCMs were cultured on glass coverslips in a 24‐well plate. For LKB1 analysis, NRCMs were treated with CTRP9 (1 µg/mL) for 0, 7.5 and 15 minutes. Cells were incubated overnight at 4°C with primary antibodies for LKB1 (Santa Cruz Biotechnology) in PBS with 0.1% Triton X‐100 in a humidified chamber. Cells were washed with PBS and incubated with secondary antibody (1:200 dilution; Cell Signaling Technology) for 30 minutes at 37°C. Fluorescent images were acquired by laser scanning confocal microscopy (LSM710; Zeiss).
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