Rat sicam 1

Blood glucose was measured using an Accu-Chek™ Performa glucometer (Roche, Basel, Switzerland), and urine volume was measured gravimetrically. The concentrations of rat sVCAM-1 (MyBioSource, San Diego, CA, USA, Cat. No. MBS762680), rat sICAM-1 (MyBioSource, San Diego, CA, USA, Cat. No. MBS266128), rat albumin (AssayMax, St. Charles, MO, USA, Cat. No. ERA3201-1), rat VEGF-A (Thermo Scientific, Waltham, MA, USA, Cat. No. ERVEGFA), rat thrombin–antithrombin (TAT) complex (Novus Biologicals, Easter Ave Centennial, CO, USA, Cat. No. NBP2-68132) and nucleosomes (Roche, Basel, Switzerland, Cat. No 11 774 425 001) were measured using immunoenzymatic assays. The enzymatic method was used to measure creatinine (Wiener lab., Rosario, Santa Fe, Argentina) and urea (BioSystems, Barcelona, Spain) concentrations. Total protein was measured with a biuret reagent.

The blood glucose was determined with an Accu-Chek Performa glucometer (Roche, Basel, Switzerland), and the urine volume was determined gravimetrically. Immunoenzymatic assays were used to measure the concentration of rat sVCAM-1 (MyBioSource, San Diego, CA, USA, Cat. No. MBS762680) and rat sICAM-1 (MyBioSource, San Diego, CA, USA, Cat. No. MBS266128). The creatinine (Wiener lab., Rosario, Santa Fe, Argentina) and urea (BioSystems, Barcelona, Spain) concentrations were measured by the enzymatic method.