Gentamicin

Manufactured by Atlanta Biologicals

Gentamicin is a broad-spectrum antibiotic used in laboratory settings. It is effective against a wide range of Gram-negative and some Gram-positive bacteria. Gentamicin functions by inhibiting protein synthesis in bacterial cells, ultimately leading to cell death.

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Lab products found in correlation

Lb agar, by Merck Group (1 mentions) L proline, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Enzyme linked immunosorbent assay elisa kit, by R&D Systems (1 mentions) Phorbol 12 myristate13 acetate, by Avantor (1 mentions) Allevyn adhesive, by Smith & Nephew (1 mentions) Cellsens imaging software, by Olympus (1 mentions)

2 protocols using gentamicin

Bacterial Growth and Analysis Protocol

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Lysogeny broth (LB) with 5% NaCl, LB agar, KCN, sodium succinate, sodium acetate, sodium citrate, L-proline, L-glutamine, glucose and potassium nitrate were purchased from Sigma. Other reagents include Muller Hinton Broth Agar (Fluka), M9 powdered salts (BD), gentamicin (Atlanta Biologicals), Breath-Easy Membranes (Diversified BD Biotech), XTT (2,3-Bis-2-Methoxy-4-Nitro-5-Sulfophenl-2-Tetrazolium-5-Carboxanilide from Caymen chemicals) and nicotinamide adenine dinucleotide (NADH from Caymen chemicals).

Torres A., Kasturiarachi N., DuPont M., Cooper V.S., Bomberger J, & Zemke A. (2019). NADH Dehydrogenases in Pseudomonas aeruginosa Growth and Virulence. Frontiers in Microbiology, 10, 75.

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Characterization of [CEL + CS] Composite Materials

Cited 1 time

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The preparation and complete characterization of the [CEL + CS] composite materials used in this study was described in our previous publication.^{20 (link)} Minimal essential medium (MEM), 10% Fetal Bovine Serum (FBS), and gentamicin were obtained from Atlanta Biologicals, Lawrenceville, GA. CellTiter 96^® aqueous non-radioactive cell proliferation assay was obtained from Promega, Madison, WI. One Percent Penicillin–Streptomycin and phorbol 12-myristate 13-acetate (PMA) were obtained from VWR, Radnor, PA. The enzyme-linked immunosorbent assay (ELISA) kit was from R&D Systems, Minneapolis, MN and was used according to the manufacturer’s instructions. Images of the fibroblasts and macrophages were taken with an Olympus microscopic camera using CellSens Imaging Software (Olympus, Center Valley, PA). Kendall – Curity AMD gauze sponges, 8 and 12 ply (Covidien, Mansfield, MA), Promogran matrix wound dressing (Johnson & Johnson, New Brunswick, NJ), Aquacel and DuoDERM CGF (ConvaTec, Princeton, NJ), Allevyn adhesive (Smith + Nephew, Andover, MA) are wound dressing that were purchased commercially.

Harkins A.L., Duri S., Kloth L.C, & Tran C.D. (2014). Chitosan–cellulose composite for wound dressing material. Part 2. Antimicrobial activity, blood absorption ability, and biocompatibility. Journal of biomedical materials research. Part B, Applied biomaterials, 102(6), 1199-1206.

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