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Actinomycin

Manufactured by Thermo Fisher Scientific
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Actinomycin is a laboratory reagent used in various scientific applications. It is a polypeptide antibiotic that inhibits DNA-dependent RNA synthesis. Actinomycin is commonly used in cell and molecular biology research.

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Lab products found in correlation

Anti mouse gr1 fitc, by AnaSpec (2 mentions) Anti mouse f4 80 pe, by AnaSpec (2 mentions) Anti mouse cd11b apc, by AnaSpec (2 mentions) Anti mouse cd45 pacific blue, by AnaSpec (2 mentions) Facscanto 2, by BD (2 mentions) Facsdiva software, by BD (2 mentions)

Spelling variants of this product

Actinomycin D (110 citations) 7-amino-actinomycin (8 citations) 7-amino-actinomycin D (7-AAD) staining solution (4 citations) 7AAD (7-amino-actinomycin D; (2 citations) 7-amino-actinomycin-D solution (7AAD, (2 citations) 7-amino-actinomycin D (AAD; (2 citations)

2 protocols using actinomycin

1

Splenic Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions from spleens were stained with the following antibodies (eBioscience, San Diego, CA) or flourescent dyes at 1:100 dilutions unless stated: anti-mouse GR1 FITC, anti-mouse F4/80 PE, anti-mouse CD11b APC, anti-mouse CD45 Pacific Blue, and 7AAD (1:500, AnaSpec Inc., Fremont, CA). Cells were fixed with 1% Paraformaldehyde (PFA) for 20 min with Actinomycin (ACROS Organics, Geel, Belgium). Stained cells were analyzed on a BD FACSCantoII using BD FACS DIVA software (BD Bioscience, San Jose, CA).
Symington J.W., Wang C., Twentyman J., Boaitey N.O., Schwendener R., Núñez G., Schilling J.D, & Mysorekar I.U. (2015). ATG16L1 deficiency in macrophages drives clearance of Uropathogenic E. coli in an IL-1β dependent manner. Mucosal immunology, 8(6), 1388-1399.
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2

Splenic Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions from spleens were stained with the following antibodies (eBioscience, San Diego, CA) or flourescent dyes at 1:100 dilutions unless stated: anti-mouse GR1 FITC, anti-mouse F4/80 PE, anti-mouse CD11b APC, anti-mouse CD45 Pacific Blue, and 7AAD (1:500, AnaSpec Inc., Fremont, CA). Cells were fixed with 1% Paraformaldehyde (PFA) for 20 min with Actinomycin (ACROS Organics, Geel, Belgium). Stained cells were analyzed on a BD FACSCantoII using BD FACS DIVA software (BD Bioscience, San Jose, CA).
Symington J.W., Wang C., Twentyman J., Boaitey N.O., Schwendener R., Núñez G., Schilling J.D, & Mysorekar I.U. (2015). ATG16L1 deficiency in macrophages drives clearance of Uropathogenic E. coli in an IL-1β dependent manner. Mucosal immunology, 8(6), 1388-1399.
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