Analysis camera control software

Manufactured by Olympus

AnalySIS camera control software is a product that enables the control and management of Olympus digital cameras used in laboratory and research applications. The software provides fundamental functionalities for camera operation, image capture, and data management. It serves as an interface between the camera hardware and the user, facilitating the acquisition and organization of visual data.

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Lab products found in correlation

Tecnai 12 transmission electron microscope, by Thermo Fisher Scientific (3 mentions) Megaview 3 ccd camera, by Olympus (3 mentions) Gatan 626 cryo holder, by Ametek (1 mentions) Formvar carbon coated 400 mesh copper grids, by Agar Scientific (1 mentions) Eagle 4k 4k ccd camera, by Thermo Fisher Scientific (1 mentions) 541 filter paper, by Cytiva (1 mentions)

Close spelling variants of this product

AnalySIS v3.2 camera control software (4 citations)

4 protocols using analysis camera control software

Cryo-TEM Imaging of Hydrated Samples

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A laboratory-built humidity-controlled
vitrification system was
used to prepare the samples for cryo-TEM measurements. Humidity was
kept close to 80% for all experiments and the ambient temperature
was 295 K. Aliquots (3 μL) of each sample were pipetted onto
a 300-mesh copper grid coated with lacey Formvar over a perforated
carbon support (ProSciTech, Thurwingowa, Australia GSCu300FL-50C)
that were glow discharged in nitrogen to render them hydrophilic.
After ∼10 s adsorption time the grid was blotted manually with
Whatman 541 filter paper for ∼2 s. Blotting time was optimized
for each sample. The grid was then plunged into liquid ethane cooled
by liquid nitrogen. Frozen grids were stored in liquid nitrogen until
the cryo-TEM measurements were performed. The samples were examined
using a Gatan 626 cryoholder (Gatan, Pleasanton, CA) and Tecnai 12
Transmission Electron Microscope (FEI, Eindhoven, The Netherlands)
at an operating voltage of 120 kV. At all times, low dose procedures
were followed using an electron dose of 8–10 electrons Å^–2. Images were recorded using a Megaview III CCD camera
and AnalySIS camera control software (Olympus). Consistent images
were observed in at least three independent grid squares.

Clulow A.J., Parrow A., Hawley A., Khan J., Pham A.C., Larsson P., Bergström C.A, & Boyd B.J. (2017). Characterization of Solubilizing Nanoaggregates Present in Different Versions of Simulated Intestinal Fluid. The Journal of Physical Chemistry. B, 121(48), 10869-10881.

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Transmission Electron Microscopy of Extracellular Vesicles

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EV samples were prepared for transmission electron microscopy on formvar carbon-coated 400-mesh copper grids (Agar Scientific, Stansted, United Kingdom) which were treated with plasma to render the carbon surface hydrophilic. EVs (3 μL) were placed onto a carbon grid and incubated for 20 min for adsorption, with the grids rinsed 3 times in Sorenson’s buffer and fixed with 1% (w/v) glutaraldehyde for 5 min. Again, the grids were washed; 8 times in ultrapure water. The samples were stained using 2% (w/v) uranyl acetate (UA) for 5 min after which 0.5 µL final embedding solution (ES; 700 µL of 2% (w/v) methyl cellulose, 100 µL 3% UA, 187 µL Milli-Q water, 12.5 µL of 2% Phosphotungstic acid) was applied. Excess ES was removed, and the grids were air-dried for 15 min. The samples were analysed using a Tecnai 12 Transmission Electron Microscope, operating at an accelerating voltage of 120 kV. Images were acquired using a Megaview III CCD camera and AnalySIS camera control software (Olympus).

Kusuma G.D., Li A., Zhu D., McDonald H., Inocencio I.M., Chambers D.C., Sinclair K., Fang H., Greening D.W., Frith J.E, & Lim R. (2022). Effect of 2D and 3D Culture Microenvironments on Mesenchymal Stem Cell-Derived Extracellular Vesicles Potencies. Frontiers in Cell and Developmental Biology, 10, 819726.

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Negative Staining of Hendra Virus Nucleoprotein

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Carbon-coated 300-mesh copper grids were glow-discharged in nitrogen to render the carbon film hydrophilic. A 4 μL aliquot of each HeV N protein (0.03 mg/mL) was pipetted onto separate grids. After a 30 s adsorption time, excess liquid was drawn off using Whatman 541 filter paper, a 5 μL water wash applied, followed by staining with 2% phosphotungstic acid for 10 s. Grids were air-dried before use. The samples were examined using a Tecnai 12 Transmission Electron Microscope (FEI, Eindhoven, The Netherlands) at an operating voltage of 120 KV. Images were recorded using either a Megaview III CCD camera and AnalySIS camera control software (Olympus), or a FEI Eagle 4k × 4k CCD camera. Measurements were made using ImageJ software (http://rsb.info.nih.gov/ij/).

Pearce L.A., Yu M., Waddington L.J., Barr J.A., Scoble J.A., Crameri G.S, & McKinstry W.J. (2015). Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli. Protein Expression and Purification, 116, 19-29.

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Characterization of Retroviral Capsid Assemblies

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Pr55^Gag and Pr55^GagΔp6 were concentrated to 2.0 mg mL^-1 in 50 mM TRIS pH 8.0 containing 1.0 M NaCl and 10 mM dithiothreitol and mixed with TG30 at a nucleic acid to protein ration of 4% (w/w) prior to dialysis against 50 mM TRIS pH 8.0 containing 150 mM NaCl and 10 mM dithiothreitol overnight at 4°C. Particles were imaged using both negative stain transmission electron microscopy and cryo-electron microscopy.
For negative stain imaging, the stock solution was diluted 100-fold to give a single layer of well-separated particles in most fields of view. Carbon-coated grids were glow discharged in nitrogen prior to use to facilitate sample spreading. Aliquots of approximately 4 μL were pipetted onto each grid and allowed to settle for 30 s. Excess sample was drawn off with filter paper, and the remaining sample stained with a drop of 2% aqueous phosphotungstic acid. Again, excess liquid was drawn off with filter paper. Grids were air dried until required. Samples were examined using a Tecnai 12 Transmission Electron Microscope (FEI, Eindhoven) at an operating voltage of 120kV. Images were recorded using a Megaview III CCD camera and AnalySIS camera control software (Olympus.)
For cryo-electron microscopy, virus particles were prepared, processed and imaged as previously described [46 (link)].

Tanwar H.S., Khoo K.K., Garvey M., Waddington L., Leis A., Hijnen M., Velkov T., Dumsday G.J., McKinstry W.J, & Mak J. (2017). The thermodynamics of Pr55Gag-RNA interaction regulate the assembly of HIV. PLoS Pathogens, 13(2), e1006221.

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