Protein quantification was performed using 1D-SDS-PAGE / SYPRO® Ruby protein staining densitometry, as previously described [23 (link)]. For immunoblotting (10 μg), membranes were probed with primary antibodies [mouse anti-E-cadherin (BD Transduction Laboratories; 1:1000), mouse anti-H-Ras (Santa Cruz Biotechnology; 1:1000), rabbit anti-YBX1 (Abcam; 1:1000), mouse anti-Vimentin (Merck Millipore; 1:1000), mouse anti-β-actin (Cell Signalling Technologies; 1:2000), mouse anti-Claudin1 (Santa Cruz Biotechnology; 1:1000), rabbit anti-Snail1 (Abcam; 1:1000), rabbit anti-Twist (Santa Cruz Biotechnology; 1:1000), or mouse anti-GAPDH (Life technologies; 1:12, 000) for 1 hr at RT in TTBS (50 mM Tris, 150 mM NaCl, 0.05% (v/v) Tween 20) followed by incubation with either IRDye 800 goat anti-mouse IgG or IRDye 700 goat anti-rabbit IgG (1:15000, LI-COR Biosciences) for 1 hr at RT in TTBS. Immunoblots were imaged using the Odyssey Infrared Imaging System, (v3.0, LI-COR Biosciences, Nebraska USA).
Rabbit anti twist
Manufactured by Santa Cruz Biotechnology
Rabbit anti-Twist is an antibody produced in rabbits that specifically recognizes the Twist protein. Twist is a transcription factor involved in the regulation of embryonic development and cell differentiation. The antibody can be used to detect and study the expression and localization of Twist in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti β actin, by Cell Signaling Technology (1 mentions)
Mouse anti e cadherin, by BD (1 mentions)
Mouse anti gapdh, by Thermo Fisher Scientific (1 mentions)
Rabbit anti snail1, by Abcam (1 mentions)
Irdye 700 goat anti rabbit igg, by LI COR (1 mentions)
Irdye 800 goat anti mouse igg, by LI COR (1 mentions)
Mouse anti h ras, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti ybx1, by Abcam (1 mentions)
Mouse anti vimentin, by Merck Group (1 mentions)
Mouse anti claudin 1, by Santa Cruz Biotechnology (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
F actin, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Rabbit anti phospho s6 ser235 236, by Cell Signaling Technology (1 mentions)
Eclipse 50i epifluorescence microscope, by Nikon (1 mentions)
2 protocols using rabbit anti twist
1
Protein Quantification and Immunoblotting Protocols
2
Immunofluorescence Staining of EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were cultured on glass coverslips and fixed with 4% paraformaldehyde solution for 10 min and processed for immunofluorescent staining using primary and secondary antibodies at the following concentrations: 1:100 for rabbit anti-Twist (Santa-Cruz Biotechnology), rabbit anti-Snail1 (Santa-Cruz Biotechnology), rabbit anti-phospho-S6 (Ser235/236) (Cell Signaling), and 1:300 for anti-rabbit Alexa-488-labeled secondary antibodies. Alexa-546-labeled phalloidin (1:300) and DAPI (1:100) were used for F-actin and nuclear staining, respectively (all from Molecular Probes, Invitrogen, Cergy-Pontoise, France). Images were taken using an Eclipse 50i epi-fluorescence microscope (Nikon Instruments, Champigny sur Marne, France) with Nis Element acquisition software.
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