We used comprehensive metabolic panels to test serum samples collected in 2.5-mL Z Serum Separator Clot Activator VACUETTE Tubes (Greiner Bio-One, Monroe, NC, USA) by using a Piccolo Xpress Chemistry Analyzer and Piccolo General Chemistry 13 Panel (Abbott Point of Care, Princeton, NJ, USA). Complete blood counts were performed on whole blood collected in 1.2-mL S-Monovette K3 EDTA Tubes (Sarstedt, Nümbrecht, Germany) by using a CELL-DYN 3700 system (Abbott Point of Care).
S monovette k3 edta tubes
Manufactured by Sarstedt
Sourced in Germany
The S-Monovette K3 EDTA tubes are a type of laboratory equipment used for the collection and storage of blood samples. They are designed to contain the anticoagulant K3 EDTA, which helps to prevent the blood from clotting during the collection and storage process.
Automatically generated - may contain errors
Lab products found in correlation
Piccolo xpress chemistry analyzer, by Abbott (1 mentions)
Cell dyn 3700 system, by Abbott (1 mentions)
Coulter ac t diff hematology analyzer, by Beckman Coulter (1 mentions)
S monovette serum gel tubes, by Sarstedt (1 mentions)
Acetonitrile, by AppliChem (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Formic acid, by AppliChem (1 mentions)
Human recombinant prorenin, by Cayman Chemical (1 mentions)
Dynabeads protein g magnetic beads, by Thermo Fisher Scientific (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
4 protocols using s monovette k3 edta tubes
1
Serum and Blood Analysis Workflow
2
Human Plasma Sample Preparation
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Human plasma was obtained from a healthy male volunteer who provided a written informed consent. Blood was collected in 3 aliquots of 9.0 mL in S-Monovette K3 EDTA tubes (SARSTEDT, Nümbrecht, Germany) after 16 h of fasting. The samples were centrifuged at 2000× g first for 5 min and then again 10 min (4 °C). Plasma aliquots (100 µL) were stored at −80 °C.
3
Blood Sample Collection and PBMC Isolation
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We obtained approximately 30 ml of blood in S-Monovette K3 EDTA tubes and 7 ml in S-Monovette Serum-Gel tubes (Sarstedt). All samples were collected in the morning (8:00 a.m.). PBMCs were then isolated using a Ficoll–Hypaque gradient as described (17 (link)), aliquoted in RPMI containing 10% DMSO and 25% FCS at 1 × 107 cells/ml, gradually cooled down to −80°C in a Mr. Frosty for 18 h and stored in liquid nitrogen until assayed. A small amount of whole blood (50 µl) was used for total and differential leukocyte counts using a Coulter Ac·T Diff hematology analyzer (Beckman Coulter). All other assays were performed using cryopreserved PBMCs or serum with one subject from each group run in parallel to control systematic variation in reagents used.
4
Quantification of Human Prorenin Levels
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Human recombinant prorenin (≥85%) was supplied by Cayman Chemicals (Ann Arbor, MI, USA). TCPK-treated modified trypsin and Dynabeads ® Protein G magnetic beads were purchased from Thermo Fisher Scientific (Rockford, IL, USA). Anti-prorenin antibodies were purchased from three different vendors (Genetex, Irvine, CA, USA; R&D Systems, Minneapolis, MN, USA; Molecular Innovations, Novi, MI, USA). Ammonium bicarbonate (>99.5%) was obtained from Sigma-Aldrich (Taufkirchen, Germany). Methanol optima ® (LC-MS grade), tetrahydrofuran (HPLC grade), acetone (HPLC grade) and dimethyl sulfoxide (DMSO; p.a.) were supplied by Fisher Chemicals (Geel, Belgium). Water (LC-MS grade) was provided by Riedel-de Haen (Seelze, Germany), while formic acid (FA; 98%, p.a.) and acetonitrile (UHPLC grade) were supplied by Applichem (Darmstadt, Germany). Human blood plasma was collected from a healthy male volunteer in S-Monovette ® K3 EDTA tubes (Sarstedt, Nuembrecht, Germany).
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