The blocking device (shown in Fig. 1 a) was used, and the device-height was adjusted to 30 cm. The acrylic partition was fabricated by attaching a 6-mm thick clear acrylic plate to the blocking device. To measure the sound transmission, the source of the sound and detector were symmetrically placed 20 cm from the blocking device. The sound source was a timer alarm. The sound detector was an iPhone 7 with an iOS platform app (Sound Level Analyzer Lite (iOS version 6.0.2))16 (link). In addition, light transmission was evaluated atthe light source and detecter, which were placed at the same position of the light source and detector. The light source was an illuminator (Fiber Illuminator C-FI115, Nikon Co., Ltd.). A fluorescence spectrometer (USB4000-FL, Ocean Optics) was used as the light detector. To measure the specular reflection of the sound/light, the source and detector were placed 20 cm from the blocking device at 10°.
Usb4000 fl
Manufactured by OceanOptics
Sourced in United States
The USB4000-FL is a compact and versatile fiber optic spectrometer designed for a variety of laboratory applications. It features a 2048-element CCD-array detector and offers a wavelength range of 200-1100 nm. The spectrometer connects to a computer via a USB interface, enabling easy integration with data acquisition software.
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Lab products found in correlation
3 protocols using usb4000 fl
1
Characterizing Sound and Light Transmission through Blocking Device
2
Spectroelectrochemical Analysis of Polypyrrole-Methylene Blue
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A spectrometer USB4000-FL equipped with SpectraSuite software was purchased from Ocean Optics (Largo, FL, USA) and was used for optical measurements.
The changes of pH were followed with pH-meter ProLine Plus (Q-i-s, Oosterhout, The Netherlands) in BRB with 0.1 M KCl solution by increasing the pH with 1 M NaOH. Continuous measurements of the UV absorbance at particular wavelengths (668 nm and 750 nm) vs. time were performed at each pH value of BRB solution. Appropriate wavelengths were selected taking into account the absorbance spectra of methylene blue with the maximum at 668 nm [34 ] and of Ppy with the maximum at 750 nm [22 (link)].
Different reference electrodes were used during co-deposition of Ppy-PMB and evaluation of electrochromic response. Ag/AgCl(3M KCl) (CH Instruments, Austin, TX, USA) was used as a reference electrode and platinum wire as a counter electrode. ITO electrode modified with Ppy-PMB layer was used as a working electrode.
A potential pulse sequence (PPS) was applied to evaluate the electrochromic properties of Ppy-PMB layer. Potential pulses were +0.8 V and −0.8 V. Duration of the pulse was 10 s. Each potential was repeated 5 times.
The changes of pH were followed with pH-meter ProLine Plus (Q-i-s, Oosterhout, The Netherlands) in BRB with 0.1 M KCl solution by increasing the pH with 1 M NaOH. Continuous measurements of the UV absorbance at particular wavelengths (668 nm and 750 nm) vs. time were performed at each pH value of BRB solution. Appropriate wavelengths were selected taking into account the absorbance spectra of methylene blue with the maximum at 668 nm [34 ] and of Ppy with the maximum at 750 nm [22 (link)].
Different reference electrodes were used during co-deposition of Ppy-PMB and evaluation of electrochromic response. Ag/AgCl(3M KCl) (CH Instruments, Austin, TX, USA) was used as a reference electrode and platinum wire as a counter electrode. ITO electrode modified with Ppy-PMB layer was used as a working electrode.
A potential pulse sequence (PPS) was applied to evaluate the electrochromic properties of Ppy-PMB layer. Potential pulses were +0.8 V and −0.8 V. Duration of the pulse was 10 s. Each potential was repeated 5 times.
3
Inverse Multifractal Analysis of Tissue Refractive Index
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The spatial distribution of tissue refractive index (RI) was recorded by a differential interference contrast (DIC) microscope (Olympus IX-81, United States). At a magnification of 60×, these DIC images were recorded by a CCD camera (ORCA-ERG, Hamamatsu, 1344 × 1024 pixel dimension 6.45 μm). The elastic scattering spectra from the multiple sites of the biopsied tissue sections were recorded by the angle resolved spectral light scattering measurements (Fig. 1). In brief, light emitted from a Xe-lamp (HPX-2000, Ocean Optics, United States) was collimated by a combination of lenses and illuminated the tissue sample at the center of a goniometric arrangement (spot size ∼1-mm-diameter). The collimated scattered light from the sample was focused into a collecting fiber probe coupled to a spectrometer (USB4000FL, Ocean Optics) for wavelength resolved signal detection. The recordings of spectra were performed (360 to 800 nm) with a spectral resolution of 2.05 nm, where the angular range was kept at 10 deg to 150 deg with an interval of 10 deg. For the inverse multifractal study, the spectra were recorded at backscattering angle θ ¼ 150 deg (Fig. 3).
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