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Easy nlc 2 nano flow hplc

Manufactured by Thermo Fisher Scientific
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The EASY-nLC II is a nano-flow high-performance liquid chromatography (HPLC) system designed for the separation and analysis of small sample volumes. It provides precise and reliable separation of complex samples, making it suitable for various applications that require high-sensitivity analysis.

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Lab products found in correlation

Scaffold 4, by Proteome Software (2 mentions) Q exactive hf orbitrap mass spectrometer, by Thermo Fisher Scientific (2 mentions) Gelcode blue stain, by Thermo Fisher Scientific (2 mentions) Scaffold ptm 33, by Proteome Software (2 mentions)

Spelling variants of this product

Easy nLC (237 citations) EASY-nLC II (72 citations) Easy-nLC II HPLC (13 citations)

2 protocols using easy nlc 2 nano flow hplc

1

Mass Spectrometry Analysis of Raptor Interactome

Check if the same lab product or an alternative is used in the 5 most similar protocols
293T cells were co-transfected with HA-Raptor and Flag-LATS1/MST1. 48 hours post-transfection, cells were harvested with EBC buffer and the whole-cell lysates were collected to perform HA immunoprecipitation. The HA immunoprecipitates were then resolved on SDS-PAGE and visualized by GelCode Blue Stain (Thermo Scientific, 24590). The band containing HA-Raptor was excised and digested with trypsin. The peptide mixture was analyzed by C18 microcapillary liquid chromatography tandem mass spectrometry (LC-MS/MS) using a high-resolution hybrid Q Exactive HF Orbitrap mass spectrometer (Thermo Scientific) coupled to an EASY-nLC II nano-flow HPLC (Thermo Scientific) in positive ion mode. Higher collision energy (HCD) fragmentation spectra acquired by data dependent acquisition (DDA) in Top 8 mode were searched against the concatenated decoy Human protein database version 20171220 (UniProt) using the Mascot 2.5.1search engine. Data were interpreted and reported using Scaffold 4.6 and Scaffold PTM 33.1 software (Proteome Software). Peptides were accepted if they passed a 1.0% false discovery rate (FDR) threshold.
Gan W., Dai X., Dai X., Xie J., Yin S., Zhu J., Wang C., Liu Y., Guo J., Wang M., Liu J., Hu J., Quinton R.J., Ganem N.J., Liu P., Asara J.M., Pandolfi P.P., Yang Y., He Z., Gao G, & Wei W. (2020). LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control. Nature cell biology, 22(2), 246-256.
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2

Mass Spectrometry Analysis of Raptor Interactome

Check if the same lab product or an alternative is used in the 5 most similar protocols
293T cells were co-transfected with HA-Raptor and Flag-LATS1/MST1. 48 hours post-transfection, cells were harvested with EBC buffer and the whole-cell lysates were collected to perform HA immunoprecipitation. The HA immunoprecipitates were then resolved on SDS-PAGE and visualized by GelCode Blue Stain (Thermo Scientific, 24590). The band containing HA-Raptor was excised and digested with trypsin. The peptide mixture was analyzed by C18 microcapillary liquid chromatography tandem mass spectrometry (LC-MS/MS) using a high-resolution hybrid Q Exactive HF Orbitrap mass spectrometer (Thermo Scientific) coupled to an EASY-nLC II nano-flow HPLC (Thermo Scientific) in positive ion mode. Higher collision energy (HCD) fragmentation spectra acquired by data dependent acquisition (DDA) in Top 8 mode were searched against the concatenated decoy Human protein database version 20171220 (UniProt) using the Mascot 2.5.1search engine. Data were interpreted and reported using Scaffold 4.6 and Scaffold PTM 33.1 software (Proteome Software). Peptides were accepted if they passed a 1.0% false discovery rate (FDR) threshold.
Gan W., Dai X., Dai X., Xie J., Yin S., Zhu J., Wang C., Liu Y., Guo J., Wang M., Liu J., Hu J., Quinton R.J., Ganem N.J., Liu P., Asara J.M., Pandolfi P.P., Yang Y., He Z., Gao G, & Wei W. (2020). LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control. Nature cell biology, 22(2), 246-256.
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