Lc ms 0.1 formic

MacConkey agar media (lactose-free) was purchased from Difco. Unlabeled melibiose and 4-nitrophenyl-α-d-galactopyranoside (α-NPG) were purchased from Sigma-Aldrich. Maltose was purchased from (Acros Organics, Fisher Scientific). [1-³H]Melibiose (5.32 Ci/mmol) was custom synthesized by PerkinElmer. Detergents undecyl-β-d-maltopyranoside (UDM) and n-dodecyl-β-d-maltopyranoside (DDM) were purchased from Anatrace. E. coli lipids (Extract Polar, 100600) were purchased from Avanti Polar Lipids, Inc,. Oligodeoxynucleotides were synthesized by Integrated DNA Technologies. LC-MS grade water, LC-MS 0.1% formic, LC-MS grade acetonitrile with 0.1% formic acid in water were purchased from Fisher Scientific (Hampton, NH). Guanidine hydrochloride, citric acid, and zirconium (IV) oxide 5 μm powder were purchased from Sigma-Aldrich (St. Louis, MO). Deuterium oxide (99⁺% D) was purchased from Cambridge Isotope Laboratories (Tewksbury, MA). All other materials were reagent grade and obtained from commercial sources.

MacConkey agar media (lactose-free) was purchased from Difco. Unlabeled melibiose and 4-nitrophenyl-α-D-galactopyranoside (α-NPG) were purchased from Sigma-Aldrich. Maltose was purchased from Acros Organics (Fisher Scientific). [1-³H]Melibiose (5.32 Ci/mmol) was custom synthesized by PerkinElmer. Detergents undecyl-β-D-maltopyranoside (UDM) and n-dodecyl-β-D-maltopyranoside (DDM) were purchased from Anatrace. E. coli lipids (Extract Polar, 100600) were purchased from Avanti Polar Lipids, Inc. Oligodeoxynucleotides were synthesized by Integrated DNA Technologies. LC-MS grade water, LC-MS 0.1% formic, LC-MS grade acetonitrile with 0.1% formic acid in water were purchased from Fisher Scientific (Hampton, NH). Guanidine hydrochloride, citric acid, and Zirconium (IV) oxide 5-μm powder were purchased from Sigma-Aldrich (St. Louis, MO). Deuterium oxide (99⁺% D) was purchased from Cambridge Isotope Laboratories (Tewksbury, MA). All other materials were reagent grade and obtained from commercial sources.