Fritted ag agcl electrodes

Potassium chloride, potassium phosphate dibasic, sodium chloride, sodium phosphate monobasic, and sodium hydroxide were acquired from Fischer Scientific. 6-Mercapto-1-hexanol, phosphate-buffered saline (1× PBS, pH 7.4), and tris(2-carboxyethyl)phosphine hydrochloride (TCEP) were obtained from Sigma-Aldrich. Sulfuric acid was obtained from EMD, and fritted Ag|AgCl electrodes and platinum wire were from CH Instruments. Insulated pure gold (75 μm diameter, 64 μm insulation thickness) was purchased from A-M Systems. To fabricate working electrodes, segments of gold wire were insulated using a heat-shrink polytetrafluoroethylene insulation (PTFE, HS Sub-Lite-Wall, 0.02 in, black-opaque, Lot #17747112–3) from ZEUS and soldered to gold pins purchased from CH Instruments. All of the above materials and reagents were used as received. In all electrochemical measurements, a three-electrode setup was used consisting of Ag|AgCl reference, platinum counter, and gold working electrodes, with a GAMRY Reference 600+ potentiostat/galvanostat/ZRA.

NaCl, KCl, KH₂PO₄, NaH₂PO₄, and NaOH were acquired from Fischer Scientific (NJ, USA). 6-Ferrocenyl-1-hexanethiol, ferrocenemethanol (FcMeOH), 6-mercapto-1-hexanol, 1,6-hexanedithiol, dimethylsulfoxide (DMSO), phosphate-buffered saline, trifluoroacetic acid (TFA), and tris(2-carboxyethyl)phosphine hydrochloride (TCEP) were obtained from Sigma-Aldrich (MO, USA). Maleimide-modified methylene blue was obtained from ATTO-TEC GmbH (Siegen, Germany), ethanol was obtained from Gold Shield Distributors (CA, USA), H₂SO₄ was obtained from EMD (USA), and 2 mm gold electrodes, fritted Ag|AgCl electrodes, and platinum wire were obtained from CH Instruments (TX, USA). Microcloth (2–7/8″), 1 μm monocrystalline diamond suspension, and 0.05 μm micropolish alumina powder were obtained from Buehler (IL, USA). All were used as received. We used for all electrochemical measurements an Ag/AgCl reference, a platinum counter electrode, and a CH Instrument 660D potentiostat.

NaCl, KCl, KH₂PO₄, NaH₂PO₄, and NaOH were acquired from Fischer Scientific (NJ). H₂SO₄ was purchased from EMD (USA), and 2 mm gold electrodes, fritted Ag|AgCl electrodes, and platinum wire were from CHInstruments (TX). 6-Mercapto-1-hexanol, 11-mercapto-1-undecanol, 3-mercapto-1-propanol, tris(2-carboethyl)-phosphine hydrochloride (TCEP), cocaine, and murine monoclonal anti-FLAG M2 antibody were obtained from Sigma-Aldrich (MO). Kanamycin monosulfate was purchased from GoldBio.com (MO), and ethanol was obtained from Gold Shield Distributors (CA). 2 and 7/8″ microcloth, 1 μm monocrystalline diamond suspension, and 0.05 μm micropolish alumina powder were obtained from Buehler (IL). All were used as received.