Radioimmunoassay kit

All patients were assessed with the PSQI one day before the operation [15 (link)]. The PSQI differentiates between good sleepers (PSQI global score < 6) and poor sleepers (PSQI global score ≥ 6) with high sensitivity and specificity. The patients in this study received routine general anesthesia and surgery. No additional requirements or preoperative oral analgesics were permitted. While the patients were in the operating room, standard monitoring was performed, including evaluations of systolic blood pressure, diastolic blood pressure, heart rate, electrocardiography, blood oxygen saturation and the BIS. After venipuncture, 3 ml of venous blood was collected for plasma inflammatory cytokine measurements prior to induction. Plasma samples were used to measure the cytokine concentrations (interleukin-6 [IL-6], tumor necrosis factor-α [TNF-α], IL-1β and interferon-gamma [IFN-γ]) in all patients. Commercially available radioimmunoassay kits were used to measure the plasma concentrations of IFN-γ, IL-1β, IL-6, and TNF-α (eBioscience, Bender MedSystems GmbH, Vienna, Austria).

Blood samples were taken from the jugular vein of rats that had been anesthetized by intraperitoneal injection of 10% chloral hydrate (0.4 ml/100 g) 1 h after the last gavage (in Experiment 1) or after the vaginas of all rats had opened completely (in Experiment 2). The serum was then separated using a high-speed freezing centrifuge (15 min at −20°C at 3,000 rpm/min) (Heraeus, Germany) and stored at −80°C until being assayed. Serum LH, FSH, P4, and E2 levels were determined using radioimmunoassay kits (eBioscience, Usheshidu SA) according to the manufacturer's specifications. The sensitivities of the LH, FSH, P4, and E2 tests were 0.3 mIU/ml, 0.28 mIU/ml, 0.2 ng/l, and 1.7 pg/ml, respectively, and the intra-assay coefficients were 2.6%, 6%, 10%, and 4.5%, respectively.

The outcome variable of our study was the serum 25-hydroxyvitamin D concentration, which was assayed by commercial laboratories. According to KNHANES reports [18 ,19 ], this biomarker was assayed using a radioimmunoassay kit (BioSource, Belgium or DiaSorin Inc., Stillwater, OK, USA).