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A7257

Manufactured by Merck Group
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A7257 is a laboratory equipment product manufactured by Merck Group. It is designed to perform specific tasks related to scientific research and analysis. The core function of this product is to provide a reliable and accurate tool for conducting various experiments and measurements in a laboratory setting. No further details or interpretation about the intended use of this product are provided.

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Lab products found in correlation

Trypan blue, by Merck Group (2 mentions) M8750, by Merck Group (2 mentions) A7250, by Merck Group (2 mentions) Neomycin, by Merck Group (1 mentions)

Spelling variants of this product

Dexamethasone (D4902), insulin (I5500), 3-IsoButyl-1-MethylXanthine (IBMX, I7018), biotin (B4639), D-pantothenic acid hemicalcium salt (P5155), isoproterenol (I6504), CL-316,243 (C5976), norepinephrine (A7257), dibutyryl-cAMP (D0260), triiodothyronine (T5516), acetylcholine chloride (A2661), nicotine (N3876), (2 citations)

3 protocols using a7257

1

Methamphetamine and Norepinephrine Effects

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Reagent doses were optimized in dose-response experiments in both macrophages and BAs, to account for viability and biological effects. (+) Methamphetamine hydrochloride (M8750 – Sigma-Aldrich) was added to the culture media at 60 μM, as previously reported [6 ,7 ,23 ] and cells were harvested at indicated time-points. (−) Norepinephrine (A7257, Sigma Aldrich) was added to the cultures at 0.1 μM. N-acetyl cysteine was added at 5 pg/ml (A7250, Sigma Aldrich). ROS measurements were performed at 30 min and 24 h after stimuli. Assays were performed 24 h after stimulations unless stated in figure legends. Trypan blue (Sigma Aldrich) dye exclusion staining was performed to count cells and assess viability upon plating and retrieval. Cultures with less than 80% viability were discarded.
Sanchez-Alavez M., Bortell N., Basova L., Samad F, & Marcondes M.C. (2020). Macrophages and brown adipocytes cross-communicate to modulate a thermogenic program following methamphetamine exposure. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 37(1), 1368-1382.
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2

Methamphetamine and Norepinephrine Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagent doses were optimized in dose-response experiments in both macrophages and BAs, to account for viability and biological effects. (+) Methamphetamine hydrochloride (M8750 – Sigma-Aldrich) was added to the culture media at 60 μM, as previously reported [6 ,7 ,23 ] and cells were harvested at indicated time-points. (−) Norepinephrine (A7257, Sigma Aldrich) was added to the cultures at 0.1 μM. N-acetyl cysteine was added at 5 pg/ml (A7250, Sigma Aldrich). ROS measurements were performed at 30 min and 24 h after stimuli. Assays were performed 24 h after stimulations unless stated in figure legends. Trypan blue (Sigma Aldrich) dye exclusion staining was performed to count cells and assess viability upon plating and retrieval. Cultures with less than 80% viability were discarded.
Sanchez-Alavez M., Bortell N., Basova L., Samad F, & Marcondes M.C. (2020). Macrophages and brown adipocytes cross-communicate to modulate a thermogenic program following methamphetamine exposure. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 37(1), 1368-1382.
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3

Osteocyte Norepinephrine Response

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Primary osteocytes were treated with norepinephrine (A7257, Sigma), ICI‐118551 (1458009, Sigma) at concentrations of 50 or 100 µm and neomycin (1458009, Sigma) at 10 mm.
Guo Q., Chen N., Qian C., Qi C., Noller K., Wan M., Liu X., Zhang W., Cahan P, & Cao X. (2023). Sympathetic Innervation Regulates Osteocyte‐Mediated Cortical Bone Resorption during Lactation. Advanced Science, 10(18), 2207602.
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