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2 protocols using para nitrophenyl palmitate

1

Enzymatic Immobilization for Biocatalysis

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Lipase from Aspergillus niger, racemic ketoprofen, para-nitrophenyl palmitate, p-nitrophenol (pNPP), (3-aminopropyl)triethoxysilane (APTES), Bradford reagent used for examination of the quantity of immobilized enzyme, N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA), phosphate, acetate and TRIS buffers at various pH, and silica nanoparticles were acquired from Sigma-Aldrich (Saint Louis, MO, USA). Hexane, sodium carbonate, sodium hydrogencarbonate, and methanol were purchased from Chempur, whereas chloroform was acquired from POCH.
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2

Synthesis and Characterization of Polyurethane Derivatives

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Impranil DLN was supplied by Bayer Corporation (Germany). Poly[4-(2,2-dicyanovinyl)-N-bis(hydroxyethyl)aniline-alt-(4,4’-methylenebis(phenylisocyanate)))]urethane and poly[4-(2,2-dicyanovinyl)-N-bis(hydroxyethyl)aniline-alt-(isophroronediisocyanate)]urethane were purchased from Sigma Aldrich (France).
Tween 20, Pefabloc, para-nitrophenyl acetate (pNPA), para-nitrophenyl butyrate (pNPB), para-nitrophenyl palmitate (pNPP), para-nitrophenol (pNP), tetrahydrofuran (THF), 2-sec-butylphenol, (2-Butan-2-ylphenyl), N-methylcarbamate (fenobucarb), Ethyl 2-(4-phenoxyphenoxy)ethylcarbamate (fenoxycarb) and S-Benzyl dipropylthiocarbamate (prosulfocarb) were purchased from Sigma Aldrich (France).
AZCL-xylan (XYL), AZCL-casein (CAS), AZCL-Barley β-glucan (BGLU), AZO-CM Cellulose (CMC), AZO-Carob Galactomannan (GM) were purchased from Megazyme (Ireland).
Lysonase™ Bioprocessing Reagent was purchased from Novagen (France).
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