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Coated transwell chamber

Manufactured by Corning
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Coated transwell chambers are laboratory consumables designed for a variety of cell culture applications. They feature a permeable membrane that allows for the separation and study of different cell types within a single culture system.

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Lab products found in correlation

Matrigel, by BD (2 mentions) Sdf 1α, by R&D Systems (1 mentions) Vcam 1, by R&D Systems (1 mentions) Opti mem 1, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Matrigel, by Corning (1 mentions)

Close spelling variants of this product

Matrigel‐coated Transwell upper chambers Matrigel-uncoated and -coated transwell chambers Matrigel-coated upper chambers of Transwell inserts Matrigel-coated 24-well Transwell chambers Matrigel-coated Transwell cell culture chambers Growth Factor Reduced Matrigel-coated 8 μm PET transwell chambers Matrigel-coated transwell invasion chambers Matrigel-coated Transwell insert chambers Gelatin-coated upper 8-µm pore transwell chambers Matrigel-coated transwell chambers with 8.0 µm PET membranes

5 protocols using coated transwell chamber

1

Chemotaxis Assay for Cell Migration

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Act B, Pre-PB, and PB were sorted by flow cytometry (as described above). Next, 1 × 105 cells (100 μL) were placed in the upper compartment, and IMDM medium (200 μL) containing recombinant CXCL12 (0, 0.1 or 0.5 μg/mL) (SDF-1α; R&D Systems) was placed in the lower compartment of VCAM-1 (5 μg/mL in PBS; R&D Systems) -coated transwell chamber (5-μm pore size; Corning). After incubation at 37 °C for 4 h, cells migrating into the lower chamber were counted by collecting events for a fixed time (60 s) on a LSR Fortessa flow cytometer, and their percentage relative to the total cells was calculated.
Andreani V., Ramamoorthy S., Pandey A., Lupar E., Nutt S.L., Lämmermann T, & Grosschedl R. (2018). Cochaperone Mzb1 is a key effector of Blimp1 in plasma cell differentiation and β1-integrin function. Proceedings of the National Academy of Sciences of the United States of America, 115(41), E9630-E9639.
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2

Transwell Invasion and Migration Assay

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Cells have been resuspended in serum-free culture medium in the presence of Opti-MEMI (Invitrogen). Cells have been seeded in a matrigel (BD Biosciences, USA)-coated Transwell chamber (Corning, Corning, New York, USA). 5 × 104 cells were seeded at a density of 500 μL/well. Cells have been seeded in a culture medium provided with 10% of FBS. After a determined time period, culture medium has been removed and the cells were gently wiped with a cotton swab on the upper chamber. Cells have been fixed with 4% paraformaldehyde and stained s with crystal violet. Cells have been observed and counted under a microscope. matrigel coating was not applied in the migration experiment, and the rest of the steps were the same as the invasion. N = 3, and the data were averaged [19 (link)].
Lv C., Hu Y., Zhou X., Zhu Y., Wang J, & Zhou F. (2022). CircRNA SOD2 motivates non-small cell lungs cancer advancement with EMT via acting as microRNA-2355-5p’s competing endogenous RNA to mediate calmodulin regulated spectrin associated proteins-2. Bioengineered, 13(3), 5756-5768.
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3

Glioma Cell Migration and Invasion Assay

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Glioma cells were seeded in 100-mm diameter culture dishes and incubated overnight at 37 °C. Subsequently, cells were serum starved for 16 h at 37 °C. For pimozide studies, cells were pretreated with pimozide for one hour. Cells were then harvested and added in triplicate to collagen (Advanced BioMatrix)-coated transwell chambers (migration) or matrigel (Corning)-coated transwell chambers (invasion) according to manufacturer’s protocols and allowed to migrate in presence of 10% FBS. After incubation for 4 hours at 37 °C, non-migrated cells were scrapped off the upper side of the membrane and cells migrated to the other side of the membrane were fixed with 4% paraformaldehyde (PFA) (Affymetrix) and stained with DAPI (Invitrogen). Nuclei of migrated cells were counted in five high power fields (HPF) with a 10× objective. Data represents the average of triplicate transwells.
Roos A., Dhruv H.D., Peng S., Inge L.J., Tuncali S., Pineda M., Millard N., Mayo Z., Eschbacher J.M., Loftus J.C., Winkles J.A, & Tran N.L. (2018). EGFRvIII-Stat5 Signaling Enhances Glioblastoma Cell Migration and Survival. Molecular cancer research : MCR, 16(7), 1185-1195.
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4

Wound Healing and Cell Migration Assays

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Wound healing assay was performed to determine cell migration. In short, cells grown into confluence were pretreated with 1 μg/mL mitomycin C (Sigma, Sigma‐Aldrich, St. Louis, MO, USA) for 1 h, and then were scratched with a 200‐μL sterile pipette tip on the cell monolayer. Cell images were taken under a light microscope in the beginning and at 24 h later. For transwell assay, matrigel (BD Biosciences, San Jose, CA)‐coated transwell chambers (Corning, Tewksbury, MA) were placed in a 24‐well plate containing DMEM plus 20% FBS. Cells (2 × 104) in 200 μL serum‐free DMEM were seeded into the upper chamber. Cells invaded through the chamber membrane were fixed in 4% paraformaldehyde at RT for 20 min and then stained with 0.5% hematoxylin.
Tang Y., Zhang Y., Wang C., Sun Z., Li L., Dong J, & Zhou W. (2018). 14‐3‐3ζ binds to hepatitis B virus protein X and maintains its protein stability in hepatocellular carcinoma cells. Cancer Medicine, 7(11), 5543-5553.
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5

Transwell Invasion Assay for Cell Motility

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The invasive ability of transfected cells was assessed by the transwell invasion assay. Briefly, cells were collected and resuspended in a serum‐free medium in the matrix gel mixture (BD Biosciences) coated transwell chambers (Corning) were inserted into a 24‐well plate containing 20% serum (Gibco). After 24 h of incubation, the remaining cells on the chamber's upper surface were removed using a cotton swab, and lower surface adhered cells were fixed and stained. The number of invaded cells was evaluated under a microscope and quantified with ImageJ software.
Huang T., Chen C., Du J., Zheng Z., Ye S., Fang S, & Liu K. (2023). A tRF‐5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1. Journal of Cellular and Molecular Medicine, 27(24), 4021-4033.
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