Xcelligence real time cell analyser

The xCelligence real-time cell analyser (Roche Diagnostics GmbH, Penzberg, Germany) was used to assess cell proliferation according to manufacturer’s instructions. Fibroblasts were seeded onto the E-Plate 96 (Roche Diagnostics GmbH) wells at 2000 cells/well in normal culture medium, in quadruplicates. Adherent cell growth/density was monitored continuously for up to 5 days.

UCS and MCS were eluted in DMEM with FBS for 7 days, and these eluates were used as media for cell growth, which was recorded by the xCELLigence Real-Time Cell Analyser (Roche, Basel, Switzerland). The cells were cultured in special 96-well E-plates with gold micro-electrodes incorporated into the bottoms of the wells. The system is based on measuring the electrical impedance, which is presented as the cell index. The cell index correlates positively with the coverage of the well bottoms with cells, i.e., with the cell number and spreading, and was measured every 15 min for the first 24 h and then every 30 min for the following 6 days.