The mitochondrial membrane potential (MMP) was monitored using DiOC6, as described previously [34 (link)]. For each condition, 1 × 106 cells were incubated with 1 mL of DePsipher solution (Trevigen, Gaithersburg, MD, USA), which uses a cationic dye (5,5′6,6′-tetrachloro-1,1′,3,3′tetraethylbenzimidazolylcarbocyanine iodide). After incubation for 20 min at 37 °C in a 5% CO2 incubator, cells were washed with 1 mL of pre-warmed 1X Reaction Buffer with Stabiliser Solution and subsequently analysed using an LSR Fortessa flow cytometer (488 nmargonlaser) and the FACSuite software (BD Biosciences).
Depsipher solution
Manufactured by Bio-Techne
Sourced in United States
DePsipher solution is a laboratory reagent designed for the isolation and purification of proteins from biological samples. It provides a simple and effective method for extracting target proteins while maintaining their native structure and function.
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Lab products found in correlation
3 protocols using depsipher solution
1
Mitochondrial Membrane Potential Analysis
2
MMP Assessment by DiOC6 and DePsipher Kit
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The MMP was monitored using DiOC6, as described previously [31 (link)]. We used the DePsipher™ Kit, which uses a unique cationic dye (5,5′6,6′-tetrachloro-1,1′,3,3’tetraethylbenzimidazolylcarbocyanine iodide) to indicate the loss of MMP. For each condition, 1 × 106 cells were incubated with 1 mL of DePsipher™ solution (Trevigen, Gaithersburg, MD, USA) in a 5% CO2 incubator at 37 °C for 20 min and then washed with 1 mL of prewarmed 1X reaction buffer with stabilizer solution prior to analysis on a LSR Fortessa flow cytometer (488 nm argon laser). Data were analyzed using FACSuite.
3
Mitochondrial Membrane Potential Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mitochondrial membrane potential (MMP) was monitored using DiOC 6 , as described previously (32) .
For each condition, 1 × 10 6 cells were incubated with 1 mL of DePsipher solution (Trevigen, Gaithersburg, MD, USA), which uses a cationic dye (5,5'6,6'-tetrachloro-1,1',3,3'tetraethylbenzimidazolylcarbocyanine iodide). After incubation for 20 min at 37 °C in a 5% CO 2 incubator, cells were washed with 1 mL of prewarmed 1X Reaction Buffer with Stabiliser Solution and subsequently analysed using an LSR Fortessa ow cytometer (488 nm argon laser) and the FACSuite software (BD Biosciences).
For each condition, 1 × 10 6 cells were incubated with 1 mL of DePsipher solution (Trevigen, Gaithersburg, MD, USA), which uses a cationic dye (5,5'6,6'-tetrachloro-1,1',3,3'tetraethylbenzimidazolylcarbocyanine iodide). After incubation for 20 min at 37 °C in a 5% CO 2 incubator, cells were washed with 1 mL of prewarmed 1X Reaction Buffer with Stabiliser Solution and subsequently analysed using an LSR Fortessa ow cytometer (488 nm argon laser) and the FACSuite software (BD Biosciences).
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