2 7 difluorofluorescein diacetate dcfh da

Hesperidin and icariin were purchased from Shanghai Yuan Ye Technology Co., Ltd. (batch number B20182, B21576 Shanghai, China), with a purity greater than 98%. DMEM culture medium (batch number 11965092), fetal bovine serum (batch number 12483020), and penicillin (100 U/mL) and streptomycin (100 µg/mL) (batch number 15140122) were obtained from GIBCO (New York, NY, USA). Dimethyl sulfoxide (DMSO) (batch number 472301-500ML), dexamethasone (batch number D8041), ascorbic acid (batch number A103533), β-glycerophosphate (batch number G5422-1), Calcein (batch number C302986), AAPH (batch number ajci23234), and 2,7-difluorofluorescein diacetate (DCFH-DA) (batch number D6470), prednisolone (batch number P0180), etidronate disodium (batch number B27158) were purchased from Beijing Chemical Factory Co., Ltd. (Beijing, China). The alkaline phosphatase assay kit and alkaline phosphatase chromogenic kit were obtained from Biyun Tian Biotechnology Co., Ltd. (batch number P0321S, C3206 Shanghai, China). The NO, TNF-α, and IL-6 enzyme-linked immunosorbent assay kits were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (batch number Shanghai, China).

Hesperidin and icariin were purchased from Shanghai Yuan Ye Technology Co., Ltd. (batch number B20182, B21576 Shanghai, China), with a purity greater than 98%. DMEM culture medium (batch number 11965092), fetal bovine serum (batch number 12483020), and penicillin (100 U/mL) and streptomycin (100 µg/mL) (batch number 15140122) were obtained from GIBCO (New York, NY, USA). Dimethyl sulfoxide (DMSO) (batch number 472301-500ML), dexamethasone (batch number D8041), ascorbic acid (batch number A103533), β-glycerophosphate (batch number G5422-1), Calcein (batch number C302986), AAPH (batch number ajci23234), and 2,7-difluorofluorescein diacetate (DCFH-DA) (batch number D6470), prednisolone (batch number P0180), etidronate disodium (batch number B27158) were purchased from Beijing Chemical Factory Co., Ltd. (Beijing, China). The alkaline phosphatase assay kit and alkaline phosphatase chromogenic kit were obtained from Biyun Tian Biotechnology Co., Ltd. (batch number P0321S, C3206 Shanghai, China). The NO, TNF-α, and IL-6 enzyme-linked immunosorbent assay kits were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (batch number Shanghai, China).