Fasted (8–10 h) blood samples were collected from all participants, enzymatic colorimetric assay was used to measure fasting plasma glucose (FPG) (Hexokinase activity assay, Abcam), total cholesterol (Cholesterol Gen.2, Roche), high density lipoproteins (HDLC) cholesterol (LDL-Cholesterol plus 2nd generation, Roche), triglycerides (Triglycerides/Glycerol Blanked, Roche) concentrations using Dimension Autoanalyzer (Cobas501, Roche, Switzerland). Low-density lipoprotein (LDLC) cholesterol serum concentration was calculated with Friedewald’s formula. Basal Insulin was analyzed by an immunoradiometric assay (BioSource International, Camarillo, CA, USA) in a Beckman Coulter (Fullerton, CA, USA). The homeostatic model assessment for insulin resistance index was calculated using the formula: HOMA-IR = fasting serum insulin (Fins, mU/L) *fasting plasma glucose (FPG, mmol/L) /22.5.
Dimension autoanalyzer
Manufactured by Roche
Sourced in Switzerland
The Dimension Autoanalyzer is a clinical chemistry analyzer that performs automated analysis of various analytes in biological samples, such as blood, serum, or urine. It is designed for high-throughput, accurate, and reliable testing in clinical laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Immunoradiometric assay, by Thermo Fisher Scientific (2 mentions)
Hexokinase activity assay, by Abcam (2 mentions)
Triglycerides glycerol blanked, by Roche (2 mentions)
Ldl cholesterol plus 2nd generation, by Roche (2 mentions)
Cholesterol gen 2, by Roche (2 mentions)
Irisin elisa kit ek 067 52, by Phoenix Pharmaceuticals (1 mentions)
2 protocols using dimension autoanalyzer
1
Cardiometabolic Risk Biomarkers in Fasting Plasma
2
Serum Irisin and Metabolic Markers
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Participants underwent blood sampling for the assessment of blood chemistry and hormonal parameters. The quantitative measurement of irisin in human serum samples was performed using a commercial enzyme linked immunosorbent assay (ELISA) kit (Irisin ELISA Kit EK-067-52; Phoenix Pharmaceuticals Inc., CA) according to the manufacturer’s instructions. Basal insulin was analyzed by an immunoradiometric assay (BioSource International, Camarillo, CA, USA) in a Beckman Coulter (Fullerton, CA, USA). Enzymatic colorimetric assay was used to measure fasting plasma glucose (FPG) (Hexokinase activity assay, Abcam), total cholesterol (Cholesterol Gen.2, Roche), high-density lipoproteins (HDL) cholesterol (LDL-Cholesterol plus 2nd generation, Roche), triglycerides (Triglycerides/Glycerol Blanked, Roche) concentrations using a Dimension Autoanalyzer (Cobas501, Roche, Switzerland). The homeostatic model assessment index was calculated following the formulas: HOMA-IR = fasting serum insulin (FINS, mU/L) × fasting plasma glucose (FPG, mmol/L)/22.5; HOMA-IS = 1/(FPG (mmol/L) × FINS (mIU/L)). Low-density lipoprotein (LDL) cholesterol serum concentration was calculated with Friedewald’s formula.
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