The following antibodies were used for immunofluorescence on brain sections and immunoblots: Glial Fibrillary Acidic Protein (GFAP) (1:500; mouse monoclonal; clone G‐A‐5, G3893, Sigma‐Aldrich); CD68 (1:500; rat monoclonal; clone FA‐11 (MCA1957, AbD Serotec), Calbindin D‐28 K (1:1000, mouse monoclonal; CB‐955, ascites fluid; Sigma‐Aldrich), Myelin Basic Protein (MBP) (1:1000; rabbit polyclonal; GTX22404, GeneTex), LAMP1 (1:500; rat monoclonal; clone 1D4B, Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA, USA) and p62/SQSTM1 (rabbit polyclonal, BML‐PW9860; Enzo). Fluorophore‐conjugated secondary antibodies against the corresponding primary antibody species (AlexaFluor 488, AlexaFluor 594 and AlexaFluor 647) were purchased from Invitrogen/Molecular Probes and were diluted 1:500. Analytical grade chemicals were purchased, if not stated otherwise, from Sigma‐Aldrich (MO, USA).
Cb 955
Manufactured by Merck Group
Sourced in United States
CB-955 is a laboratory instrument designed for performing high-precision analytical measurements. It features advanced technologies to ensure accurate and reliable results. The core function of CB-955 is to provide precise data analysis capabilities for scientific and research applications. Further details on its intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
Fluorophore conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Clone 1d4b, by Developmental Studies Hybridoma Bank (1 mentions)
Clone g a 5, by Merck Group (1 mentions)
Calbindin d28k, by Merck Group (1 mentions)
Bml pw9860, by Enzo Life Sciences (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647, by Thermo Fisher Scientific (1 mentions)
Ac 15, by Merck Group (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Chemidoc xr, by Bio-Rad (1 mentions)
2 protocols using cb 955
1
Immunofluorescence and Immunoblotting of Brain Tissue
2
Quantification of Kidney Protein Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total kidney protein lysates were prepared as described previously [25 (link)] and 30 μg protein was loaded to either 8% (w/v) or 12% (w/v) SDS-PAGE gel for NCX1 and CaBP28k, respectively and blotted to a PVDF membrane (Millipore, Billerica, MA, USA). Immunoblots were incubated overnight at 4°C either with mouse anti-CaBP28k (1:5,000, clone CB-955, Sigma-Aldrich, Zwijndrecht, The Netherlands), mouse anti-NCX1 (1:500, ab6495, Abcam) or mouse anti-beta-actin (1:10,000, clone AC-15, Sigma-Aldrich). Immunoblots were enhanced using chemiluminescence (ECL, Pierce, Etten-Leur, The Netherlands) and analyzed using a ChemiDoc XRS (Bio-Rad) system. Semi-quantification was performed as described previously [26 (link)].
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